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Communication Dans Un Congrès Année : 2013

Analysis of 5-methyl cytosine and 5-hydromethyl cytosine contents and distribution in different lineages during the bovine development

Résumé

DNA methylation of cytosines (5-mC) plays a crucial role in the epigenetic regulation of gene expression throughout development. The pattern of 5-mC is accurately established by de novo DNA methyltransferases (DNMT3a-3b) and inherited through mitosis by a maintenance DNA methyltransferase (DNMT1). Recently, another DNA modification, 5-hydroxymethylcytosine (5-hmC), was reported. 5-hmC is generated from 5-mC by TET proteins, and could be an intermediate in demethylation pathways. This study aims to determine the tissue- and cell- content of the 5-mC and 5-hmC amount by different approaches in mouse and in bovine species. Given the potential role of 5-hmC in nuclear reprogramming, it is crucial to obtain data in species with longer developmental time courses than the mouse.
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Dates et versions

hal-01019198 , version 1 (07-07-2014)

Identifiants

  • HAL Id : hal-01019198 , version 1
  • PRODINRA : 253375

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Hélène Jammes, Audrey Prézelin, Michel Guillomot, Anne Gabory, Juliette Salvaing, et al.. Analysis of 5-methyl cytosine and 5-hydromethyl cytosine contents and distribution in different lineages during the bovine development. Conférence J. Monod, "DNA methylation and demethylation", Centre National de la Recherche Scientifique (CNRS). FRA. Institut National de la Santé et de la Recherche Médicale (INSERM)., Sep 2013, Roscoff, France. 1 p. ⟨hal-01019198⟩
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