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Communication Dans Un Congrès Année : 2013

Investigation of inter-individual epigenetic variability in bovine clones: a high throughput study

Hélène Kiefer

Résumé

Reprogramming the differentiated cell to totipotency can be obtained following the introduction of its nucleus into an enucleated oocyte, a procedure known as cloning. We are using cattle clones as a pertinent model to assess the inter-individual epigenetic variability and its consequences on phenotypes, including agronomical relevant traits and developmental pathologies. Indeed, the developmental defects frequently associated with cloning could be related to the insufficient extent of reprogramming, leading to perturbations of the nuclear microenvironment of the early embryo with long term consequences. To identify epigenomic patterns affected by incomplete reprogramming, we used immunoprecipitation of methylated DNA followed by hybridization on a new bovine promoter microarray (MeDIP-chip). The microarray targets the upstream region of 21416 bovine genes (UMD3.1 assembly,-2000 bp to +1360 bp relative to the transcription startsite). We first focused on liver, because overgrowth of this organ is often observed in clones. The microarray has been hybridized with MeDIP samples from livers of normal Holstein animals (4 perinatal controls and 8 adults, obtained by artificial insemination and all healthy) and livers of Holstein clones (7 perinatal clones, either stillborn or suffering from severe pathologies, and 7 adult clones, with normal to pathological phenotypes). After normalization of the data, enriched probes were identified using ChIPmix (Martin-Magniette et al., Bioinformatics (2008) 24, i181) and located on the Ensembl Genome Browser. Results of exploratory analysis, including correlation clustering, Principal Component Analysis (PCA) and Independent Component Analysis (ICA), will be presented. A statistical test based on differences in the spatial distribution of the enriched probes revealed that most of the analysed promoters exhibit a clustered distribution of the enriched probes. This local enrichment shows inter-individual variability for some of the promoters, which are currently being identified and validated.
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Dates et versions

hal-01004781 , version 1 (11-06-2014)

Identifiants

  • HAL Id : hal-01004781 , version 1
  • PRODINRA : 226481

Citer

Hélène Kiefer. Investigation of inter-individual epigenetic variability in bovine clones: a high throughput study. 1. Annual Epiconcept Conference, Epigenetics and Periconception Environment, European Cooperation in Science and Technology (COST). BEL., Apr 2013, Antalya, Turkey. pp.23. ⟨hal-01004781⟩
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