Development of an Efficient In Vivo System (P-junc-TpaseIS(1223)) for Random Transposon Mutagenesis of Lactobacillus casei - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Applied and Environmental Microbiology Année : 2012

Development of an Efficient In Vivo System (P-junc-TpaseIS(1223)) for Random Transposon Mutagenesis of Lactobacillus casei

Résumé

The random transposon mutagenesis system P-junc-TpaseIS(1223) is composed of plasmids pVI129, expressing IS1223 transposase, and pVI110, a suicide transposon plasmid carrying the P-junc sequence, the substrate of the IS1223 transposase. This system is particularly efficient in Lactobacillus casei, as more than 10,000 stable, random mutants were routinely obtained via electroporation.

Dates et versions

hal-01004149 , version 1 (11-06-2014)

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Helene H. Licandro-Seraut, Sophie S. Brinster, Maarten van de Guchte, Helene H. Scornec, Emmanuelle Maguin, et al.. Development of an Efficient In Vivo System (P-junc-TpaseIS(1223)) for Random Transposon Mutagenesis of Lactobacillus casei. Applied and Environmental Microbiology, 2012, 78 (15), pp.5417 - 5423. ⟨10.1128/AEM.00531-12⟩. ⟨hal-01004149⟩
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