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Article Dans Une Revue Biochimica et Biophysica Acta Proteins and Proteomics Année : 2013

Transcription regulators controlled by interaction with enzyme IIB components of the phosphoenolpyruvate:sugar phosphotransferase system

Résumé

Numerous bacteria possess transcription activators and antiterminators composed of regulatory domains phosphorylated by components of the phosphoenolpyruvate:sugar phosphotransferase system (PTS). These domains, called PTS regulation domains (PRDs), usually contain two conserved histidines as potential phosphorylation sites. While antiterminators possess two PRDs with four phosphorylation sites, transcription activators contain two PRDs plus two regulatory domains resembling PTS components (EIIA and EIIB). The activity of these transcription regulators is controlled by up to five phosphorylations catalyzed by PTS proteins. Phosphorylation by the general PTS components El and HPr is usually essential for the activity of PRD-containing transcription regulators, whereas phosphorylation by the sugar-specific components ERA or EIIB lowers their activity. For a specific regulator, for example the Bacillus subtilis mtl operon activator MtIR, the functional phosphorylation sites can be different in other bacteria and consequently the detailed mode of regulation varies. Some of these transcription regulators are also controlled by an interaction with a sugar-specific EIIB PTS component. The EIIBs are frequently fused to the membrane-spanning EIIC and EIIB-mediated membrane sequestration is sometimes crucial for the control of a transcription regulator. This is also true for the Escherichia coli repressor Mlc, which does not contain a PRD but nevertheless interacts with the EIIB domain of the glucose-specific PTS. In addition, some PRD-containing transcription activators interact with a distinct EIIB protein located in the cytoplasm. The phosphorylation state of the EIIB components, which changes in response to the presence or absence of the corresponding carbon source, affects their interaction with transcription regulators. This article is part of a Special Issue entitled: Inhibitors of Protein Kinases (2012). (C) 2013 Elsevier B.V. All rights reserved.

Dates et versions

hal-01001483 , version 1 (04-06-2014)

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Philippe P. Joyet, Houda H. Bouraoui, Moussan M. Ake, Meriem M. Derkaoui, Arthur A. Zebre, et al.. Transcription regulators controlled by interaction with enzyme IIB components of the phosphoenolpyruvate:sugar phosphotransferase system. Biochimica et Biophysica Acta Proteins and Proteomics, 2013, 1834 (7), pp.1415 - 1424. ⟨10.1016/j.bbapap.2013.01.004⟩. ⟨hal-01001483⟩
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