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Article Dans Une Revue Micron Année : 2013

Realistic representation of Bacillus subtilis biofilms architecture using combined microscopy (CLSM, ESEM and FESEM)

Résumé

In this contribution, we used a set of microscopic techniques including confocal laser scanning microscopy (CLSM), environmental scanning electron microscopy (ESEM) and field emission scanning electron microscopy (FESEM) to analyze the three-dimensional spatial arrangement of cells and their surrounding matrix in Bacillus subtilis biofilm. The combination of the different techniques enabled a deeper and realistic deciphering of biofilm architecture by providing the opportunity to overcome the limits of each single technique. (C) 2013 Elsevier Ltd. All rights reserved.

Dates et versions

hal-01001074 , version 1 (04-06-2014)

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Arnaud A. Bridier, Thierry T. Meylheuc, Romain Briandet. Realistic representation of Bacillus subtilis biofilms architecture using combined microscopy (CLSM, ESEM and FESEM). Micron, 2013, 48, pp.65 - 69. ⟨10.1016/j.micron.2013.02.013⟩. ⟨hal-01001074⟩
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