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Article Dans Une Revue Plasmonics Année : 2014

Carbohydrate-lectin interaction on graphene-coated surface plasmon resonance (SPR) interfaces

Résumé

The paper describes the detection of carbohydrate- lectin interaction on graphene-on-metal surface plasmon resonance (SPR) interfaces. Graphene-coated gold-based SPR interfaces were formed through the transfer of large-area graphene grown by chemical vapor deposition (CVD) on polycrystalline Cu foils. The method allowed successful transfer of single- and double-layered graphene sheets onto the SPR interfaces in a reproducible manner. Functionalization of the graphene interface with mannose was achieved by simple immersion into a mannose aqueous solution (100 mM), resulting in noncovalent interactions between the aromatic ring structure of graphene and mannose. The utility of the carbohydrate-modified graphene-on-gold interface for the selective and sensitive detection of carbohydrate-lectin interactions was demonstrated using model lectins from Lens culinaris (LC) and Triticum vulgaris (TV). While LC lectin binds specifically to mannopyranoside units, TV lectin has an affinity for N-acetyl glucosamine and sialic acid residues.
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Dates et versions

hal-00994787 , version 1 (22-05-2014)

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Abra Penezic, Geetanjali Deokar, Dominique Vignaud, Emmanuelle Pichonat, Henri Happy, et al.. Carbohydrate-lectin interaction on graphene-coated surface plasmon resonance (SPR) interfaces. Plasmonics, 2014, 9, pp.677-683. ⟨10.1007/s11468-014-9686-3⟩. ⟨hal-00994787⟩
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