G4 motifs affect origin positioning and efficiency in two vertebrate replicators. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue EMBO Journal Année : 2014

G4 motifs affect origin positioning and efficiency in two vertebrate replicators.

Résumé

DNA replication ensures the accurate duplication of the genome at each cell cycle. It begins at specific sites called replication origins. Genome-wide studies in vertebrates have recently identified a consensus G-rich motif potentially able to form G-quadruplexes (G4) in most replication origins. However, there is no experimental evidence to demonstrate that G4 are actually required for replication initiation. We show here, with two model origins, that G4 motifs are required for replication initiation. Two G4 motifs cooperate in one of our model origins. The other contains only one critical G4, and its orientation determines the precise position of the replication start site. Point mutations affecting the stability of this G4 in vitro also impair origin function. Finally, this G4 is not sufficient for origin activity and must cooperate with a 200-bp cis-regulatory element. In conclusion, our study strongly supports the predicted essential role of G4 in replication initiation.

Dates et versions

hal-00987430 , version 1 (06-05-2014)

Identifiants

Citer

Anne-Laure Valton, Vahideh Hassan-Zadeh, Ingrid Lema, Nicole Boggetto, Patrizia Alberti, et al.. G4 motifs affect origin positioning and efficiency in two vertebrate replicators.. EMBO Journal, 2014, 33 (7), pp.732-46. ⟨10.1002/embj.201387506⟩. ⟨hal-00987430⟩
126 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More