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Article Dans Une Revue Nucleic Acids Research Année : 2013

Physico-chemical foundations underpinning microarray and next-generation sequencing experiments.

Andrew Harrison
  • Fonction : Auteur
Hans Binder
  • Fonction : Auteur
Conrad J Burden
  • Fonction : Auteur
Enrico Carlon
  • Fonction : Auteur
Cynthia Gibas
  • Fonction : Auteur
Lara J Gamble
  • Fonction : Auteur
Jef Hooyberghs
  • Fonction : Auteur
David P Kreil
  • Fonction : Auteur
Rastislav Levicky
  • Fonction : Auteur
Peter A Noble
  • Fonction : Auteur
Albrecht Ott
  • Fonction : Auteur
B Montgomery Pettitt
  • Fonction : Auteur
Diethard Tautz
  • Fonction : Auteur
Alexander E Pozhitkov
  • Fonction : Auteur

Résumé

Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput technologies such as microarrays and, in some cases, next-generation sequencing (NGS). A physical understanding of the hybridization process helps to determine the accuracy of these technologies. The goal of a widespread research program is to develop reliable transformations between the raw signals reported by the technologies and individual molecular concentrations from an ensemble of nucleic acids. This research has inputs from many areas, from bioinformatics and biostatistics, to theoretical and experimental biochemistry and biophysics, to computer simulations. A group of leading researchers met in Ploen Germany in 2011 to discuss present knowledge and limitations of our physico-chemical understanding of high-throughput nucleic acid technologies. This meeting inspired us to write this summary, which provides an overview of the state-of-the-art approaches based on physico-chemical foundation to modeling of the nucleic acids hybridization process on solid surfaces. In addition, practical application of current knowledge is emphasized.

Domaines

Biomatériaux

Dates et versions

hal-00973375 , version 1 (04-04-2014)

Identifiants

Citer

Andrew Harrison, Hans Binder, Arnaud Buhot, Conrad J Burden, Enrico Carlon, et al.. Physico-chemical foundations underpinning microarray and next-generation sequencing experiments.. Nucleic Acids Research, 2013, 41 (5), pp.2779-96. ⟨10.1093/nar/gks1358⟩. ⟨hal-00973375⟩
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