Development and properties of genetically encoded pH sensors in plants. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Frontiers in Plant Science Année : 2013

Development and properties of genetically encoded pH sensors in plants.

Résumé

Fluorescent proteins (FPs) have given access to a large choice of live imaging techniques and have thereby profoundly modified our view of plant cells. Together with technological improvement of imaging, they have opened the possibility to monitor physico-chemical changes within cells. For this purpose, a new generation of FPs has been engineered. For instance, pHluorin, a point mutated version of green fluorescent protein, allows to get local pH estimates. In this paper, we will describe how genetically encoded sensors can be used to measure pH in the microenvironment of living tissues and subsequently discuss the role of pH in (i) exocytosis, (ii) ion uptake by plant roots, (iii) cell growth, and (iv) protein trafficking.
Fichier principal
Vignette du fichier
2013_Martiniere_Frontiers in Plant science_Development and properties of genetically encoded ph sensors in plants_1.pdf (227.62 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-00932235 , version 1 (29-05-2020)

Identifiants

Citer

Alexandre Martinière, Guilhem Desbrosses, Hervé Sentenac, Nadine Paris. Development and properties of genetically encoded pH sensors in plants.. Frontiers in Plant Science, 2013, 4, pp.523. ⟨10.3389/fpls.2013.00523⟩. ⟨hal-00932235⟩
131 Consultations
172 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More