Article Dans Une Revue Veterinary Research Année : 2004

Immunophenotypic characterization and depletion of pulmonary intravascular macrophages of horses

Om Parbhakar
  • Fonction : Auteur
Tanya Duke
  • Fonction : Auteur
Hugh Townsend
  • Fonction : Auteur
Baljit Singh
  • Fonction : Auteur

Résumé

- Pulmonary intravascular macrophages (PIMs) are present in horses and are believed to increase their sensitivity to endotoxin-induced cardio-pulmonary shock. However, owing to a lack of a marker for PIMs and the inability to isolate them, their precise contributions in the horse remain unknown. We designed this study to identify an immuno-phenotypic marker for PIMs and to develop a protocol for their transient depletion with gadolinium chloride (GC). GC is a lanthanide that has been used to deplete liver and lung macrophages. The horses (N = 15) were divided into control (n = 5) and GC-treated (n = 10) groups and the lung samples were examined by routine and immunocytochemical light and electron microscopy. GC-treated horses were euthanized at 48 h (n = 6) and 72 h (n = 4) post-treatment. The PIMs reacted with MAC-387 but not with ED-1 and CD-68 anti-macrophage antibodies. GC reduced the number of PIMs in horses at 48 and 72 h compared with the control (p < 0.05). There were increased intravascular TUNEL-positive cells in GC-treated horses and electron microscopy showed apoptotic PIMs in these horses. These data show that MAC-387 is a reliable marker for PIMs and GC is a safe tool to reduce the number of PIMs.

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hal-00902815 , version 1 (11-05-2020)

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Om Parbhakar, Tanya Duke, Hugh Townsend, Baljit Singh. Immunophenotypic characterization and depletion of pulmonary intravascular macrophages of horses. Veterinary Research, 2004, 35 (1), pp.39-51. ⟨10.1051/vetres:2003041⟩. ⟨hal-00902815⟩
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