Substitution of the $\alpha $-lactalbumin transcription unit by a CAT cDNA within a BAC clone silenced the locus in transgenic mice without affecting the physically linked Cyclin T1 gene - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Genetics Selection Evolution Année : 2003

Substitution of the $\alpha $-lactalbumin transcription unit by a CAT cDNA within a BAC clone silenced the locus in transgenic mice without affecting the physically linked Cyclin T1 gene

Solange Soulier
  • Fonction : Auteur
Marthe Hudrisier
  • Fonction : Auteur
José Costa da Silva
  • Fonction : Auteur
Caroline Maeder
  • Fonction : Auteur
Céline Viglietta
  • Fonction : Auteur
Nathalie Besnard
Jean-Luc Vilotte

Résumé

We recently reported that a goat bacterial artificial chromosome (BAC) clone conferred site-independent expression in transgenic mice of the two loci present within its insert, the ubiquitously expressed Cyclin T1 and the mammary specific $\alpha$-lactalbumin ($\alpha$lac) genes. To assess if this vector could target mammary-restricted expression of cDNA, the CAT ORF was introduced by homologous recombination in Escherichia coli in place of the $\alpha$lac transcription unit. The insert of this modified BAC was injected into mice and three transgenic lines were derived. None of these lines expressed the CAT gene suggesting that the use of long genomic inserts is not sufficient to support the expression of intron-less transgenes. The physically linked goat Cyclin T1 locus was found to be active in all three lines. This observation reinforced the hypothesis that the two loci are localised in two separate chromatin domains.
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hal-00894444 , version 1 (11-05-2020)

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Solange Soulier, Marthe Hudrisier, José Costa da Silva, Caroline Maeder, Céline Viglietta, et al.. Substitution of the $\alpha $-lactalbumin transcription unit by a CAT cDNA within a BAC clone silenced the locus in transgenic mice without affecting the physically linked Cyclin T1 gene. Genetics Selection Evolution, 2003, 35 (2), pp.239-247. ⟨10.1051/gse:2003006⟩. ⟨hal-00894444⟩
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