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Article Dans Une Revue Analytical Chemistry Année : 2013

Use of limonene in countercurrent chromatography: a green alkane substitute.

Résumé

Counter-current chromatography (CCC) is a preparative separation technique working with the two liquid phases of a biphasic liquid system. One phase is used as the mobile phase when the other, the stationary phase, is held in place by centrifugal fields. Limonene is a biorenewable cycloterpene solvent coming from orange peel waste. It was evaluated as a possible substitute for heptane in CCC separations. The limonene/methanol/water and heptane/methanol/water phase diagrams are very similar at room temperature. The double bonds of the limonene molecule allows for possible π-π interactions with solutes rendering limonene slightly more polar than heptane giving small differences in solute partition coefficients in the two systems. The 24% higher limonene density is a difference with heptane that has major consequences in CCC. The polar and apolar phases of the limonene/methanol/water 10/9/1 v/v have -0.025 g/cm(3) density difference (lower limonene phase) compared to +0.132 g/cm(3) with heptane (upper heptane phase). This precludes the use of this limonene system with hydrodynamic CCC columns that need significant density difference to retain a liquid stationary phase. It is an advantage with hydrostatic CCC columns because density difference is related to the working pressure drop: limonene allows one to work with high centrifugal fields and moderate pressure drop. Limonene has the capability to be a "green" alternative to petroleum-based solvents in CCC applications.
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Dates et versions

hal-00876113 , version 1 (23-10-2013)

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Citer

Karine Faure, Elodie Bouju, Pauline Suchet, Alain Berthod. Use of limonene in countercurrent chromatography: a green alkane substitute.. Analytical Chemistry, 2013, 85 (9), pp.4644-4650. ⟨10.1021/ac4002854⟩. ⟨hal-00876113⟩
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