This study presents the results of calibration/validation (C/V) of Envisat satellite radar altimeter over Lake Issykkul located in Kyrgyzstan, which was chosen as a dedicated radar altimetry C/V site in 2004. The objectives are to estimate the absolute altimeter bias of Envisat and its orbit based on cross-over analysis with TOPEX/Poseidon (T/P), Jason-1 and Jason-2 over the ocean. We have used a new method of GPS data processing in a kinematic mode, developed at the Groupe de Recherche de Geodesie Spatiale (GRGS), which allows us to calculate the position of the GPS antenna without needing a GPS reference station. The C/V is conducted using various equipments: a local GPS network, a moving GPS antenna along the satellites tracks over Lake Issykkul, In Situ level gauges and weather stations. The absolute bias obtained for Envisat from field campaigns conducted in 2009 and 2010 is between 62.1 and 63.4 ± 3.7 cm, using the Ice-1 retracking algorithm, and between 46.9 and 51.2 cm with the ocean retracking algorithm. These results differ by about 10 cm from previous studies, principally due to improvement of the C/V procedure. Apart from the new algorithm for GPS data processing and the orbit error reduction, more attention has been paid to the GPS antenna height calculation, and we have reduced the errors induced by seiche over Lake Issykkul. This has been assured using cruise data along the Envisat satellite track at the exact date of the pass of the satellite for the two campaigns. The calculation of the Envisat radar altimeter bias with respect to the GPS levelling is essential to allow the continuity of multi-mission data on the same orbit, with the expected launch of SARAL/Altika mission in 2012. Implications for hydrology in particular, will be to produce long term homogeneous and reliable time series of lake levels worldwide.