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Article Dans Une Revue Carbohydrate Polymers Année : 2013

Activity of chitin deacetylase from Colletotrichum gloeosporioides on chitinous substrates

Neith Pacheco
  • Fonction : Auteur
Laurent David
Keiko Shirai
  • Fonction : Auteur

Résumé

Production of chitin deacetylases from the phytopathogenic fungus Colletotrichum gloeosporioides was successfully achieved by submerged fermentation. The highest specific activity of 0.018 U mg(-1) of protein was obtained after 96 h of cultivation at pH 6 and 28 degrees C. Two bands with molecular weights of 35 kDa and 170 kDa determined with SDS-PAGE displayed deacetylase activities as detected in the zymograms. Reacetylated commercial chitosan (52% acetylation degree) was used as substrate for the extracellular crude extract in order to estimate the kinetic parameters of acetate production as undirected deacetylation measurement. The highest acetate production of 12.8 mu mol mL(-1) was obtained using 7.5 mg mL(-1) of substrate. The produced enzyme from C. gloeosporioides achieved up to 25% deacetylation of a chitin substrate (hydrolyzed biological chitin) having 80% degree of acetylation, M-W of 102 x 10(3) g mol(-1) and a crystallinity index of ca. 60%.

Dates et versions

hal-00868072 , version 1 (01-10-2013)

Identifiants

Citer

Neith Pacheco, Stéphane Trombotto, Laurent David, Keiko Shirai. Activity of chitin deacetylase from Colletotrichum gloeosporioides on chitinous substrates. Carbohydrate Polymers, 2013, 96, pp.227-232. ⟨10.1016/j.carbpol.2013.03.051⟩. ⟨hal-00868072⟩
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