Piezo-electrically driven mechanical stimulation of sensory neurons. - Archive ouverte HAL Access content directly
Journal Articles Methods in Molecular Biology Year : 2013

Piezo-electrically driven mechanical stimulation of sensory neurons.

Abstract

Mechanotransduction, the conversion of a mechanical stimulus into a biological response, constitutes the basis of a variety of physiological functions such as the senses of touch, balance, proprioception, blood pressure, and hearing. In vertebrates, mechanosensation is mediated by mechanosensory neurons, whose cell bodies are located in trigeminal and dorsal root ganglia. Here, we describe an in vitro model of mechanotransduction that provides an opportunity to explore the properties of mechanosensitive channels in mammalian sensory neurons. The mechano-clamp method allows applying local force on plasma membrane of whole-cell patch-clamped sensory neurons. This technique uses a mechanical probe driven by a computer-assisted piezoelectric microstage to repeatedly stimulate sensory neurons with accurate control of stimulus strength, duration, and speed.
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Dates and versions

hal-00855019 , version 1 (28-08-2013)

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Jizhe Hao, Jérôme Ruel, Bertrand Coste, Yann Roudaut, Marcel Crest, et al.. Piezo-electrically driven mechanical stimulation of sensory neurons.. Methods in Molecular Biology, 2013, 998, pp.159-70. ⟨10.1007/978-1-62703-351-0_12⟩. ⟨hal-00855019⟩
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