Detection of an estrogen derivative in two breast cancer cell lines using a single core multimodal probe for imaging (SCoMPI) imaged by a panel of luminescent and vibrational techniques. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Analyst Année : 2013

Detection of an estrogen derivative in two breast cancer cell lines using a single core multimodal probe for imaging (SCoMPI) imaged by a panel of luminescent and vibrational techniques.

Christophe Sandt
Slavka Kascakova
Alexandre Dazzi
Anne Vessières

Résumé

3-Methoxy-17α-ethynylestradiol or mestranol is a prodrug for ethynylestradiol and the estrogen component of some oral contraceptive formulations. We demonstrate here that a single core multimodal probe for imaging - SCoMPI - can be efficiently grafted onto mestranol allowing its tracking in two breast cancer cell lines, MDA-MB-231 and MCF-7 fixed cells. Correlative imaging studies based on luminescence (synchrotron UV spectromicroscopy, wide field and confocal fluorescence microscopies) and vibrational (AFMIR, synchrotron FTIR spectromicroscopy, synchrotron-based multiple beam FTIR imaging, confocal Raman microspectroscopy) spectroscopies were consistent with one another and showed a Golgi apparatus distribution of the SCoMPI-mestranol conjugate in both cell lines.
Fichier principal
Vignette du fichier
Clède_2013_Detection_of_an.pdf (501.12 Ko) Télécharger le fichier
Origine : Publication financée par une institution
Loading...

Dates et versions

hal-00849792 , version 1 (23-02-2015)

Identifiants

Citer

Sylvain Clède, François Lambert, Christophe Sandt, Slavka Kascakova, Miriam Unger, et al.. Detection of an estrogen derivative in two breast cancer cell lines using a single core multimodal probe for imaging (SCoMPI) imaged by a panel of luminescent and vibrational techniques.. Analyst, 2013, 138 (19), pp.5627-5638. ⟨10.1039/c3an00807j⟩. ⟨hal-00849792⟩
703 Consultations
296 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More