Quantitative one- and two-dimensional (13)C spectra of microcrystalline proteins with enhanced intensity. - Archive ouverte HAL Access content directly
Journal Articles Journal of Biomolecular NMR Year : 2013

Quantitative one- and two-dimensional (13)C spectra of microcrystalline proteins with enhanced intensity.

Abstract

We recorded quantitative, uniformly enhanced one- and two-dimensional (13)C spectra of labelled microcrystalline proteins. The approach takes advantage of efficient equilibration of magnetization by low-power proton irradiation using Phase Alternated Recoupling Irradiation Schemes and benefits simultaneously from uniform sensitivity enhancement due to efficient spin exchange that can overcome T1((13)C) constraints and the presence of heteronuclear Overhauser effects.

Dates and versions

hal-00841177 , version 1 (04-07-2013)

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Rudra N Purusottam, Geoffrey Bodenhausen, Piotr Tekely. Quantitative one- and two-dimensional (13)C spectra of microcrystalline proteins with enhanced intensity.. Journal of Biomolecular NMR, 2013, 57 (1), pp.11-19. ⟨10.1007/s10858-013-9759-1⟩. ⟨hal-00841177⟩
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