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Communication Dans Un Congrès Année : 2013

Removal of living cells from biosensing surfaces in droplet-based microfluidics using surface acoustic waves

Résumé

Removal of living biological cells from surfaces is a critical process for many applications in the area of biosensing and lab-on-a-chip. Trypsin is one of the most effective biochemical tools used to cleave the cells proteins that are responsible for bonding cells to surfaces [K. A. Walsh, Meth. Enzymol. 19, 41 (1970)]. We propose a method using Rayleigh-type (20 MHz) surface acoustic wave (SAW)-based mixing [Renaudin et al., Lab Chip 10, 111 (2010)] as an accelerator for trypsin-mediated removal of living cells from surfaces. In the experiments, a 10 μL droplet of Hank’s Balanced Salt Solution (HBSS)-trypsin is placed on a piezoelectric substrate covered with human embryonic kidney cells (HEK293). Using phase contrast microscopy, cells removal time for different acoustic power levels and trypsin concentrations is measured. Results from validation experiments show that a minimum of 180 s is necessary to completely release surface-bonded cells covered by the 10 µL droplet without the use of SAW (negative control). By using microstreaming flow in the droplets generated by the SAW, cells are released from the surface in less than 8 s. This work will contribute to improved lab-on-a-chip devices based on living cell biosensing
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Dates et versions

hal-00823442 , version 1 (17-05-2013)

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Adrien Bussonnière, Alan Renaudin, Yannick Miron, Michel Grandbois, Michael Baudoin, et al.. Removal of living cells from biosensing surfaces in droplet-based microfluidics using surface acoustic waves. 21st International Congress on Acoustics, ICA 2013, 165th Meeting of the Acoustical Society of America, 52nd Meeting of the Canadian Acoustical Association, Jun 2013, Montréal, QC, Canada. ⟨10.1121/1.4805347⟩. ⟨hal-00823442⟩
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