<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-00820790</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-25T01:03:29+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">In vitro activation of the NADPH oxidase by fluoride. Possible involvement of a factor activating GTP hydrolysis on Rac (Rac-GAP).</title>
            <author role="aut">
              <persName>
                <forename type="first">J.</forename>
                <surname>Wölfl</surname>
              </persName>
              <idno type="halauthorid">709229-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">M. C.</forename>
                <surname>Dagher</surname>
              </persName>
              <idno type="halauthorid">414295-0</idno>
              <affiliation ref="#struct-586"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Alexandra</forename>
                <surname>Fuchs</surname>
              </persName>
              <email type="md5">4bbb9818f431e2ba5ca0d641423b1320</email>
              <email type="domain">cea.fr</email>
              <idno type="idhal" notation="string">alexandra-fuchs</idno>
              <idno type="idhal" notation="numeric">749944</idno>
              <idno type="halauthorid" notation="string">53788-749944</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-5536-8716</idno>
              <idno type="GOOGLE SCHOLAR">https://scholar.google.com/citations?user=8RV_9s8AAAAJ&amp;hl=en</idno>
              <affiliation ref="#struct-586"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">M.</forename>
                <surname>Geiszt</surname>
              </persName>
              <idno type="halauthorid">709207-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">E.</forename>
                <surname>Ligeti</surname>
              </persName>
              <idno type="halauthorid">709209-0</idno>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Marie-Claire</forename>
                <surname>Dagher</surname>
              </persName>
              <email type="md5">16a92a66fdebde7a6e37680303039cc5</email>
              <email type="domain">ibs.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2013-05-06 16:17:01</date>
              <date type="whenModified">2025-12-18 15:08:08</date>
              <date type="whenReleased">2013-05-06 16:17:01</date>
              <date type="whenProduced">1996-07-15</date>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="140374">
                <persName>
                  <forename>Marie-Claire</forename>
                  <surname>Dagher</surname>
                </persName>
                <email type="md5">16a92a66fdebde7a6e37680303039cc5</email>
                <email type="domain">ibs.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-00820790</idno>
            <idno type="halUri">https://hal.science/hal-00820790</idno>
            <idno type="halBibtex">wolfl:hal-00820790</idno>
            <idno type="halRefHtml">&lt;i&gt;European Journal of Biochemistry&lt;/i&gt;, 1996, 239 (2), pp.369-75</idno>
            <idno type="halRef">European Journal of Biochemistry, 1996, 239 (2), pp.369-75</idno>
            <availability status="restricted"/>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="CEA">CEA - Commissariat à l'énergie atomique</idno>
            <idno type="stamp" n="UGA">HAL Grenoble Alpes</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="UNIV-GRENOBLE1">Université Joseph Fourier - Grenoble I</idno>
            <idno type="stamp" n="BIG" corresp="CEA">Institut de Biosciences et Biotechnologies de Grenoble</idno>
            <idno type="stamp" n="TEST-UGA">TEST-UGA</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">In vitro activation of the NADPH oxidase by fluoride. Possible involvement of a factor activating GTP hydrolysis on Rac (Rac-GAP).</title>
                <author role="aut">
                  <persName>
                    <forename type="first">J.</forename>
                    <surname>Wölfl</surname>
                  </persName>
                  <idno type="halauthorid">709229-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">M. C.</forename>
                    <surname>Dagher</surname>
                  </persName>
                  <idno type="halauthorid">414295-0</idno>
                  <affiliation ref="#struct-586"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Alexandra</forename>
                    <surname>Fuchs</surname>
                  </persName>
                  <email type="md5">4bbb9818f431e2ba5ca0d641423b1320</email>
                  <email type="domain">cea.fr</email>
                  <idno type="idhal" notation="string">alexandra-fuchs</idno>
                  <idno type="idhal" notation="numeric">749944</idno>
                  <idno type="halauthorid" notation="string">53788-749944</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-5536-8716</idno>
                  <idno type="GOOGLE SCHOLAR">https://scholar.google.com/citations?user=8RV_9s8AAAAJ&amp;hl=en</idno>
                  <affiliation ref="#struct-586"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">M.</forename>
                    <surname>Geiszt</surname>
                  </persName>
                  <idno type="halauthorid">709207-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">E.</forename>
                    <surname>Ligeti</surname>
                  </persName>
                  <idno type="halauthorid">709209-0</idno>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">160012</idno>
                <idno type="issn">0014-2956</idno>
                <idno type="eissn">1432-1033</idno>
                <title level="j">European Journal of Biochemistry</title>
                <imprint>
                  <publisher>Febs Press</publisher>
                  <biblScope unit="volume">239</biblScope>
                  <biblScope unit="issue">2</biblScope>
                  <biblScope unit="pp">369-75</biblScope>
                  <date type="datePub">1996-07-15</date>
                </imprint>
              </monogr>
              <idno type="pubmed">8706742</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <classCode scheme="mesh">Animals</classCode>
              <classCode scheme="mesh">Enzyme Activation</classCode>
              <classCode scheme="mesh">NADPH Oxidase</classCode>
              <classCode scheme="mesh">Neutrophils</classCode>
              <classCode scheme="mesh">Proteins</classCode>
              <classCode scheme="mesh">Sodium Fluoride</classCode>
              <classCode scheme="mesh">Superoxides</classCode>
              <classCode scheme="mesh">Swine</classCode>
              <classCode scheme="mesh">Thionucleotides</classCode>
              <classCode scheme="mesh">Time Factors</classCode>
              <classCode scheme="mesh">GTP-Binding Proteins</classCode>
              <classCode scheme="mesh">GTPase-Activating Proteins</classCode>
              <classCode scheme="mesh">Guanosine 5'-O-(3-Thiotriphosphate)</classCode>
              <classCode scheme="mesh">Guanosine Diphosphate</classCode>
              <classCode scheme="mesh">Guanosine Triphosphate</classCode>
              <classCode scheme="mesh">Hydrolysis</classCode>
              <classCode scheme="mesh">Kinetics</classCode>
              <classCode scheme="mesh">NADH, NADPH Oxidoreductases</classCode>
              <classCode scheme="halDomain" n="sdv.imm.ii">Life Sciences [q-bio]/Immunology/Innate immunity</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>The possible mechanism of activation of the NADPH oxidase by fluoride was investigated in the cell-free system. It is shown that the stimulatory effect of fluoride is inhibited by guanosine 5'-O-(2-thiodiphosphate) (GDP[S]) and potentiated by GTP. The effect of fluoride is not additive with GTP[S]. Fluoride activation requires the presence of Mg2+ in millimolar concentration but is independent of Al3+. The activating effect of fluoride is preserved in solubilized membrane extract after removal of the majority of heterotrimeric GTP-binding proteins by immunoadsorption. Fluoride has no direct action either on the nucleotide exchange of GTP hydrolysis of the isolated Rac protein. In contrast, fluoride effectively inhibits Rac-GTPase activity enhanced by a membrane component. In this way, fluoride could prolong the prevalence of Rac in the GTP-bound state and, as a consequence, activate NADPH oxidase. The possibility of the involvement of a membrane-bound Rac GTPase-activating protein activity in the physiological regulation of the enzyme is raised.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-586" status="OLD">
          <orgName>Biochimie et biophysique des systèmes intégrés</orgName>
          <orgName type="acronym">BBSI</orgName>
          <date type="end">2007</date>
          <desc>
            <address>
              <addrLine>17 Rue des martyrs 38054 GRENOBLE CEDEX 9</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www-dsv.cea.fr/thema/bbsi/Accueil.htm</ref>
          </desc>
          <listRelation>
            <relation active="#struct-51016" type="direct"/>
            <relation name="DSV/IRTSV" active="#struct-300016" type="direct"/>
            <relation name="UMR5092" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-51016" status="OLD">
          <idno type="IdRef">026404796</idno>
          <idno type="ROR">https://ror.org/02aj0kh94</idno>
          <orgName>Université Joseph Fourier - Grenoble 1</orgName>
          <orgName type="acronym">UJF</orgName>
          <date type="end">2015-12-31</date>
          <desc>
            <address>
              <addrLine>BP 53 - 38041 Grenoble Cedex 9</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.ujf-grenoble.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-300016" status="VALID">
          <idno type="IdRef">026372061</idno>
          <idno type="ISNI">0000000122998025</idno>
          <idno type="ROR">https://ror.org/00jjx8s55</idno>
          <idno type="Wikidata">Q868550</idno>
          <orgName>Commissariat à l'énergie atomique et aux énergies alternatives</orgName>
          <orgName type="acronym">CEA</orgName>
          <desc>
            <address>
              <addrLine>Centre de SaclayCentre de GrenobleCentre de Cadaracheetc</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.cea.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>