Competitive binding of UBPY and ubiquitin to the STAM2 SH3 domain revealed by NMR - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue FEBS Letters Année : 2012

Competitive binding of UBPY and ubiquitin to the STAM2 SH3 domain revealed by NMR

Résumé

To date, the signal transducing adaptor molecule 2 (STAM2) was shown to harbour two ubiquitin binding domains (UBDs) known as the VHS and UIM domains, while the SH3 domain of STAM2 was reported to interact with deubiquitinating enzymes (DUBs) like UBPY and AMSH. In the present study, NMR evidences the interaction of the STAM2 SH3 domain with ubiquitin, demonstrating that SH3 constitutes the third UBD of STAM2. Furthermore, we show that a UBPY-derived peptide can outcompete ubiquitin for SH3 binding and vice versa. These results suggest that the SH3 domain of STAM2 plays versatile roles in the context of ubiquitin mediated receptor sorting.

Dates et versions

hal-00819142 , version 1 (30-04-2013)

Identifiants

Citer

Anja Lange, Mouhamad-Baligh Ismail, Gwladys Rivière, Maggy Hologne, Denis Lacabanne, et al.. Competitive binding of UBPY and ubiquitin to the STAM2 SH3 domain revealed by NMR. FEBS Letters, 2012, 586 (19), pp.3379-3384. ⟨10.1016/j.febslet.2012.07.047⟩. ⟨hal-00819142⟩
88 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More