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Article Dans Une Revue ChemBioChem Année : 2013

The addressing fragment of mitogaligin: first insights into functional and structural properties.

Violette Senille
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Françoise Paquet
Norbert Garnier
Ned Lamb
Alain Legrand
Agnès F Delmas
Céline Landon

Résumé

Mitogaligin is a mitochondrion-targeting protein involved in cell death. The sequence of the protein is unrelated to that of any known pro- or antiapoptotic protein. Mitochondrial targeting is controlled by an internal sequence from residues 31 to 53, and although this sequence is essential and sufficient to provoke cell death, the precise mechanism of action at the mitochondrial membrane remains to be elucidated. Here, by focusing on the [31-53] fragment, we first assessed and confirmed its cell cytotoxicity by microinjection. Subsequently, with the aid of membrane models, we evaluated the impact of the membrane environment on the 3D structure of the peptide and on how the peptide is embedded and oriented within membranes. The fragment is well organized, even though it does not contain a canonical secondary structure, and adopts an interfacial location. Structural comparison with other membrane-interacting Trp-rich peptides demonstrated similarities with the antimicrobial peptide tritrpcidin.

Dates et versions

hal-00817503 , version 1 (24-04-2013)

Identifiants

Citer

Violette Senille, Dominique Lelievre, Françoise Paquet, Norbert Garnier, Ned Lamb, et al.. The addressing fragment of mitogaligin: first insights into functional and structural properties.. ChemBioChem, 2013, 14 (6), pp.711-20. ⟨10.1002/cbic.201200715⟩. ⟨hal-00817503⟩
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