N-glycosylation modification of proteins is an early marker of the enterocytic differentiation process of HT-29 cells. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Reproduction Nutrition Development Année : 1990

N-glycosylation modification of proteins is an early marker of the enterocytic differentiation process of HT-29 cells.

Résumé

The human colon cancer cell line HT-29 remains totally undifferentiated when glucose is present in the culture medium (HT-29 Glc+), while the same cells may undergo typical enterocytic differentiation after reaching confluence when grown in glucose-deprived medium (HT-29 Glc-). Recently, we demonstrated a deficiency in the overall N-glycan processing in confluent undifferentiated cells, whereas differentiated cells follow a classical pattern of N-glycosylation. The main changes in N-glycosylation observed in confluent undifferentiated cells may be summarised as follows: 1) the conversion of high mannose into complex glycopeptides is greatly decreased; 2) this decreased conversion could be a consequence of an accumulation of Man9-8-GlcNAc2-Asn high mannose species. Whether these changes in N-glycan processing appear progressively during cell culture or are already present from the beginning of the culture was investigated in this study by comparing the actual status of N-glycan processing in exponentially growing HT-29 Glc- and HT-29 Glc+ cells. Under these conditions, HT-29 Glc- cells do not exhibit any characteristics of differentiation. The conversion of high mannose into complex glycoproteins is severely reduced in HT-29 Glc+ cells, regardless of the growth phase studied. In contrast, HT-29 Glc- cells display a normal pattern of N-glycan processing in both growth phases. We therefore conclude that N-glycan processing may be used as an early biochemical marker of the enterocytic differentiation process of HT-29 cells.

Domaines

Chimie organique
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Dates et versions

hal-00814789 , version 1 (17-04-2013)

Identifiants

  • HAL Id : hal-00814789 , version 1
  • PUBMED : 2397029

Citer

E. Ogier-Denis, C. Bauvy, M. Aubery, P. Codogno, Christian Sapin, et al.. N-glycosylation modification of proteins is an early marker of the enterocytic differentiation process of HT-29 cells.. Reproduction Nutrition Development, 1990, 30 ((3)), pp.325-30. ⟨hal-00814789⟩
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