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Article Dans Une Revue Molecular Oral Microbiology Année : 2013

Treponema denticola improves adhesive capacities of Porphyromonas gingivalis.

Résumé

Porphyromonas gingivalis, an important etiological agent of periodontal disease, is frequently found associated with Treponema denticola, an anaerobic spirochete, in pathogenic biofilms. However, interactions between these two bacteria are not well understood at the molecular level. In this study, we seek to link the influence of T. denticola on the expression of P. gingivalis proteases with its capacities to adhere and to form biofilms. The P. gingivalis genes encoding Arg-gingipain A (RgpA), Lys-gingipain (Kgp), and hemagglutinin A (HagA) were more strongly expressed after incubation with T. denticola compared with P. gingivalis alone. The amounts of the three resulting proteins, all of which contain hemagglutinin adhesion domains, were increased in culture supernatants. Moreover, incubation of P. gingivalis with T. denticola promoted static and dynamic biofilm formation, primarily via a time-dependent enhancement of P. gingivalis adhesion capacities on bacterial partners such as Streptococcus gordonii. Adhesion of P. gingivalis to human cells was also increased. These results showed that interactions of P. gingivalis with other bacterial species, such as T. denticola, induce increased adhesive capacities on various substrata by hemagglutinin adhesion domain-containing proteins.

Dates et versions

hal-00799040 , version 1 (11-03-2013)

Identifiants

Citer

Vincent Meuric, Bénédicte Martin, Hélène Guyodo, Astrid Rouillon, Zohreh Tamanai-Shacoori, et al.. Treponema denticola improves adhesive capacities of Porphyromonas gingivalis.. Molecular Oral Microbiology, 2013, 28 (1), pp.40-53. ⟨10.1111/omi.12004⟩. ⟨hal-00799040⟩
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