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Communication Dans Un Congrès Lab on a Chip Année : 2012

Dielectrophoretic separation of blood pathogens ou Bacterial extraction from biological samples using DEP forces

Résumé

We report here a method based on DEP separation to concentrate pathogens out of a biological sample by combining positive and negative DEP to separate pathogens from the sample matrix. In this approach, we take advantage from the large tolerance of micro-organisms towards osmotic shock to perform dielectrophoretic separation in a low electric conductivity medium. This condition enables to collect micro-organisms by positive DEP, while lysed blood cells are repelled from the electrodes by negative DEP. Our microfluidic device is designed based on numerical simulation. This approach is validated on a large range of microorganisms, since it has been tested with different species of bacteria (S. epidermidis (Gram +) and E. coli (Gram -)) and yeasts (C. albicans).
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Dates et versions

hal-00786577 , version 1 (01-09-2013)

Identifiants

  • HAL Id : hal-00786577 , version 1

Citer

Emilie Bisceglia, Myriam Cubizolles, Frédéric Mallard, Olivier Français, Bruno Le Pioufle. Dielectrophoretic separation of blood pathogens ou Bacterial extraction from biological samples using DEP forces. lab on a chip world congress, Sep 2012, San Diego, United States. pp.1. ⟨hal-00786577⟩
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