Molecular cloning of actin genes in Trichomonas vaginalis and phylogeny inferred from actin sequences. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue FEMS Microbiology Letters Année : 1997

Molecular cloning of actin genes in Trichomonas vaginalis and phylogeny inferred from actin sequences.

Résumé

The parasitic protozoan Trichomonas vaginalis is known to contain the ubiquitous and highly conserved protein actin. A genomic library and a cDNA library have been screened to identify and clone the actin gene(s) of T. vaginalis. The nucleotide sequence of one gene and its flanking regions have been determined. The open reading frame encodes a protein of 376 amino acids. The sequence is not interrupted by any introns and the promoter could be represented by a 10 bp motif close to a consensus motif also found upstream of most sequenced T. vaginalis genes. The five different clones isolated from the cDNA library have similar sequences and encode three actin proteins differing only by one or two amino acids. A phylogenetic analysis of 31 actin sequences by distance matrix and parsimony methods, using centractin as outgroup, gives congruent trees with Parabasala branching above Diplomonadida.

Domaines

Protistologie
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Dates et versions

hal-00783616 , version 1 (01-02-2013)

Identifiants

  • HAL Id : hal-00783616 , version 1
  • PUBMED : 9252588

Citer

Geneviève Bricheux, Guy Brugerolle. Molecular cloning of actin genes in Trichomonas vaginalis and phylogeny inferred from actin sequences.. FEMS Microbiology Letters, 1997, 153 (1), pp.205-13. ⟨hal-00783616⟩
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