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Article Dans Une Revue Journal of Separation Science Année : 2012

Modeling the electrophoresis of highly charged peptides: application to oligolysines.

Résumé

The "coarse-grained" bead modeling methodology, BMM, is generalized to treat electrostatics at the level of the nonlinear Poisson-Boltzmann equation. This improvement makes it more applicable to the important class of highly charged macroions and highly charged peptides in particular. In the present study, the new nonlinear Poisson-Boltzmann, NLPB-BMM procedure is applied to the free solution electrophoretic mobility of low molecular mass oligolysines (degree of polymerization 1-8) in lithium phosphate buffer at pH 2.5. The ionic strength is varied from 0.01 to 0.10 M) and the temperature is varied from 25 to 50°C. In order to obtain quantitative agreement between modeling and experiment, a small amount of specific phosphate binding must be included in modeling. This binding is predicted to increase with increasing temperature and ionic strength.

Dates et versions

hal-00773695 , version 1 (14-01-2013)

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Hengfu Wu, Stuart A. Allison, Catherine Perrin, Hervé Cottet. Modeling the electrophoresis of highly charged peptides: application to oligolysines.. Journal of Separation Science, 2012, 35 (4), pp.556-62. ⟨10.1002/jssc.201100873⟩. ⟨hal-00773695⟩
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