Comprehensive characterization of Annonaceous acetogenins within a complex extract by HPLC-ESI-LTQ-Orbitrap® using post-column lithium infusion. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Mass Spectrometry Année : 2012

Comprehensive characterization of Annonaceous acetogenins within a complex extract by HPLC-ESI-LTQ-Orbitrap® using post-column lithium infusion.

Jessica Le Ven
  • Fonction : Auteur
Guy Lewin
  • Fonction : Auteur
David Touboul
Pierre Champy
  • Fonction : Auteur

Résumé

Annonaceous acetogenins (AAGs) are a homogenous class of polyketides proposed as environmental neurotoxins. Previous dereplication studies of AAGs were limited by the use of low-resolution mass spectrometers. Only poor information in terms of structures was provided due to the limited fragmentation of protonated or sodium cationized species. An innovative approach, using reversed-phase high-performance liquid chromatography coupled to a hybrid linear ion trap / orbitrap mass spectrometer (LTQ-Orbitrap®), was therefore performed. Sensitivity was enhanced by post-column infusion of lithium, since AAGs have a high affinity for this cation. High level of structural information was obtained from low-energy-collision-induced dissociation fragmentation experiments of lithium-cationized AAGs ([M + Li](+) ions) as demonstrated with purified standards. The method was then applied to a total ethyl-acetate extract prepared from commercial soursop nectar (Annona muricata L.). The sensitivity, mass accuracy and specific fragmentation patterns proved to be particularly useful for characterization of the AAGs. Typical structural identification procedure and unexpected observations for specific structural types are illustrated, with major and minor compounds. Copyright © 2012 John Wiley & Sons, Ltd.

Domaines

Chimie organique

Dates et versions

hal-00758261 , version 1 (28-11-2012)

Identifiants

Citer

Jessica Le Ven, Isabelle Schmitz-Afonso, Guy Lewin, Olivier Laprévote, Alain Brunelle, et al.. Comprehensive characterization of Annonaceous acetogenins within a complex extract by HPLC-ESI-LTQ-Orbitrap® using post-column lithium infusion.. Journal of Mass Spectrometry, 2012, 47 (11), pp.1500-9. ⟨10.1002/jms.3092⟩. ⟨hal-00758261⟩
20 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More