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Communication Dans Un Congrès Année : 2009

Engineering functionalised gold and silver nanoparticles for biophotonics: robust water-soluble assemblies with integrated fluorescent modules

Résumé

Metal nanoparticles, especially those made of the noble metals gold, silver and copper, show a strong optical response which can be tailored through their shape, size and composition. An intriguing aspect of these noble metal nanostructures is their influence on the photophysics of nearby molecules, as exemplified by effects such as surface-enhanced Raman scattering or the quenching of fluorescence. The strong interaction with light makes metal nanoparticles interesting objects for application in bioanalytical detection, imaging, and possibly phototherapies. From a materials point of view it is important for biophotonic applications to make the metal particles compatible with biological media: they need to be protected against aggregation and remain intact while exposed to the myriad of biochemical species present. We have been exploring self-assembled monolayers of hydrophilic, tetraethyleneglycol-terminated ligands for the protection of gold nanoparticles.[1] These monolayers give rise to PEGylated particles that are particularly robust in aqueous solution at high ionic strengths and even extreme pHs. Recently we found that the monolayers are also very effective in protecting silver and mixed gold/silver nanoparticles. Another aspect that we have been investigating is the introduction of functional molecular modules into water-borne nanoparticle assemblies. Such modules may be fluorescent entities, which are interesting for the study of the effect of metal nanostructures on molecular fluorescence,[2] or biological moieties for biomolecular recognition. We have applied two different strategies to achieve this integration: layer-by-layer deposition on the particle surface of functionalised polyelectrolytes, and the use of functionalised disulfides for the formation of the protecting ligand mantle. We will present recent results on the preparation, protection, functionalisation and purification of gold and silver nanoparticles, as well as ensemble photophysical investigations on particles in aqueous suspension. [1] N. Nerambourg, R. Praho, M.H.V. Werts, D. Thomas, M. Blanchard-Desce, Int. J. Nanotechnol., 5, 722-740 (2008). [2] Schneider, G. Decher, N. Nerambourg, R. Praho, M.H.V. Werts, M. Blanchard-Desce, Nano Lett., 6, 530-536 (2006).
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Dates et versions

hal-00753369 , version 1 (19-11-2012)

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  • HAL Id : hal-00753369 , version 1

Citer

Martinus H. V. Werts, R. Praho, Julien R. G. Navarro. Engineering functionalised gold and silver nanoparticles for biophotonics: robust water-soluble assemblies with integrated fluorescent modules. Nanosystem Engineering and Biophotonics, Mar 2009, Cachan, France. ⟨hal-00753369⟩
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