Relevance of exocytotic glutamate release from retinal glia. - Archive ouverte HAL Access content directly
Journal Articles Neuron Year : 2012

Relevance of exocytotic glutamate release from retinal glia.

Michal Slezak
  • Function : Author
Antje Grosche
  • Function : Author
Aurore Niemiec
  • Function : Author
Naoyuki Tanimoto
  • Function : Author
Thomas A Münch
  • Function : Author
Britni Crocker
  • Function : Author
Philippe Isope
Wolfgang Härtig
  • Function : Author
Susanne C Beck
  • Function : Author
Gesine Huber
  • Function : Author
Martine Perraut
  • Function : Author
Michael Reber
  • Function : Author
Monique Miehe
  • Function : Author
Daniel Metzger
Klaudia Szklarczyk
  • Function : Author
Ryszard Przewlocki
  • Function : Author
Mathias W Seeliger
  • Function : Author
Dominique Sage-Ciocca
  • Function : Author
Sophie Reibel
  • Function : Author
Frank W Pfrieger

Abstract

Glial cells release molecules that influence brain development, function, and disease. Calcium-dependent exocytosis has been proposed as potential release mechanism in astroglia, but the physiological relevance of "gliotransmission" in vivo remains controversial. We focused on the impact of glial exocytosis on sensory transduction in the retina. To this end, we generated transgenic mice to block exocytosis by Cre recombinase-dependent expression of the clostridial botulinum neurotoxin serotype B light chain, which cleaves vesicle-associated membrane protein 1-3. Ubiquitous and neuronal toxin expression caused perinatal lethality and a reduction of synaptic transmission thus validating transgene function. Toxin expression in Müller cells inhibited vesicular glutamate release and impaired glial volume regulation but left retinal histology and visual processing unaffected. Our model to study gliotransmission in vivo reveals specific functions of exocytotic glutamate release in retinal glia.

Dates and versions

hal-00728041 , version 1 (04-09-2012)

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Michal Slezak, Antje Grosche, Aurore Niemiec, Naoyuki Tanimoto, Thomas Pannicke, et al.. Relevance of exocytotic glutamate release from retinal glia.. Neuron, 2012, 74 (3), pp.504-16. ⟨10.1016/j.neuron.2012.03.027⟩. ⟨hal-00728041⟩
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