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Article Dans Une Revue Research in Microbiology Année : 2011

The chemical-in-mu well: a high-throughput technique for identifying solutes eliciting a chemotactic response in motile bacteria

Résumé

Bacteria, and in particular marine bacteria, can be found in environments that are poor in nutrients. To survive, they are able to move toward more favorable niches by a mechanism called chemotaxis, whose first step consists in the detection of substrates by chemoreceptors. We developed a chemotactic assay enabling rapid testing of several hundred different solutes and we identified several molecules eliciting a chemotactic response from two aquatic Shewanella species. We propose that this assay be used for other bacteria to determine the repertoire of chemotactic molecules, generally not clearly elucidated. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

Dates et versions

hal-00725012 , version 1 (23-08-2012)

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Joshua Armitano, Claudine Baraquet, Valerie Michotey, Vincent Mejean, Cecile Jourlin-Castelli. The chemical-in-mu well: a high-throughput technique for identifying solutes eliciting a chemotactic response in motile bacteria. Research in Microbiology, 2011, 162, pp.934-938. ⟨10.1016/j.resmic.2011.03.001⟩. ⟨hal-00725012⟩
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