Chemical constituents of Anacolosa pervilleana and their antiviral activities. - Archive ouverte HAL Access content directly
Journal Articles Fitoterapia Year : 2012

Chemical constituents of Anacolosa pervilleana and their antiviral activities.

Mélanie Bourjot
  • Function : Author
Pieter Leyssen
  • Function : Author
Cécilia Eydoux
Jean-Claude Guillemot
  • Function : Author
Bruno Canard
Philippe Rasoanaivo
  • Function : Author
Marc Litaudon

Abstract

In an effort to identify novel inhibitors of Chikungunya (CHIKV) and Dengue (DENV) virus replication, a systematic study with 820 ethyl acetate extracts of Madagascan plants was performed in a virus-cell-based assay for CHIKV and a DENV NS5 RNA-dependant RNA polymerase (RdRp) assay. The extract obtained from the leaves of Anacolosa pervilleana was selected for its significant activity in both assays. One new (E)-tridec-2-en-4-ynedioic acid named anacolosine (1), together with three known acetylenic acids, the octadeca-9,11,13-triynoic acid (2), (13E)-octadec-13-en-9,11-diynoic acid (3), (13E)-octadec-13-en-11-ynoic acid (4), two terpenoids, lupenone (5) and β-amyrone (6), and one cyanogenic glycoside, (S)-sambunigrin (7) were isolated. Their structures were elucidated by comprehensive analyses of NMR spectroscopy and mass spectrometry data. The inhibitory potency of these compounds was evaluated on CHIKV, DENV RdRp and West-Nile polymerase virus (WNV RdRp). Both terpenoids showed a moderate activity against CHIKV (EC(50) 77 and 86μM, respectively) and the acetylenic acids produced IC(50) values around 3μM in the DENV RdRp assay.

Dates and versions

hal-00722062 , version 1 (31-07-2012)

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Mélanie Bourjot, Pieter Leyssen, Cécilia Eydoux, Jean-Claude Guillemot, Bruno Canard, et al.. Chemical constituents of Anacolosa pervilleana and their antiviral activities.. Fitoterapia, 2012, 83 (6), pp.1076-1080. ⟨10.1016/j.fitote.2012.05.004⟩. ⟨hal-00722062⟩
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