Comparison of collision-induced dissociation and electron-induced dissociation of singly charged mononucleotides
Résumé
The dissociation of singly charged mononucleotides by electron induced dissocn. (EID) and sustained off resonance irradiation/collision induced dissociation (SORI-CID) was investigated. The experiments have been carried out on deprotonated mononucleotides monomers. In general the same productions are observed in the EID and SORI-CID spectra but some additional productions have been obtained with EID. It was shown by MS3 experiments that all these addional productions have been produced through consecutive dissociations. of the [M-2H][rad] - radical anion. In addition, H/D exchange and sequential MS3 experiments demonstrated that the m/z 247 fragment ion produced in EID of deprotonated 2'-deoxyguanosine 5'-monophosphate corresponds to the association of the phosphate group and the nucleobase. They were most likely linked by a non-covalent bond that was initially present in the precursor ion.
Domaines
Chimie analytiqueOrigine | Fichiers produits par l'(les) auteur(s) |
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