Androgen-induced kidney hypertrophy in the European bullhead (Cottus sp.): a potential biomarker of androgen exposure. - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2012

Androgen-induced kidney hypertrophy in the European bullhead (Cottus sp.): a potential biomarker of androgen exposure.

Résumé

The present work was designed to identify an androgeno-regulated signal in the kidney of the European bullhead (Cottus sp.) and to characterise response patterns prior to laboratory and field applications to address androgenic potential of chemicals. In a first step, gender dependent ultrastructural modifications of kidney were quantified using the Kidney Epithelium Height (KEH) measurement. During breeding period, male bullheads exhibited an increased KEH value of 20.96 ± 3.57 μm compared to female (16.96 ± 1.28 μm). No gender difference was recorded in non-breeding bullheads. In a second step, bullheads out of breeding period were exposed 14 or 21 days to different concentrations of androgens (0.5 or 5 μg/L of trenbolone (Tb), 1 or 10 μg/L of 11-ketotestosterone (11KT), 0.5 or 5 μg/L of Spironolactone (SPI), 0.5, 5 or 50 μg/L of Dihydrotestosterone (DHT)), estrogen (0.05 or 0.5 μg/L of Ethinyl-estradiol (EE2)) or heavy metal (1 or 10 μg/L of Cadmium (Cd)). Results showed that androgens are able to induce kidney hypertrophy. Trenbolone induced a dose-dependent hypertrophy (KEH= 16.59 ± 0.81 μm at 0.5 μg/L and 21.01 ± 1.76 μm at 5 μg/L vs 15.58 ± 0.85 μm for the controls) as the higher concentration of 11-KT (KEH = 17.55 ± 1.48 μm at 10 μg/L) and DHT (17.43 ± 1.77 μm at 50 μg/L). SPI and Cd have no effect on kidney ultrastructure. In bullheads exposed to EE2, a trend to decrease of KEH was observed. In a next step, wild bullheads were electrofished in 9 streams located in the North of France and characterised by various contamination levels. KEH responses don't allow to discriminate both reference (KEH = 16.03.± 1.25 μm) and contaminated (KEH = 16.17 ± 1.08 μm) sites. This work highlights an androgen-regulated signal in the kidney of the European bullhead. However, kidney hypertrophy appears as few sensitive and time consuming indicators. Also, identification of associated protein and/or RNA modifications could provide a relevant biomarker of androgen exposure.
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Dates et versions

hal-00704593 , version 1 (05-06-2012)

Identifiants

  • HAL Id : hal-00704593 , version 1

Citer

M. Villeret, C. Blanchard, L.R. Le Rohic, Laure Wiest, Emmanuelle Vulliet, et al.. Androgen-induced kidney hypertrophy in the European bullhead (Cottus sp.): a potential biomarker of androgen exposure.. 6th SETAC World Congress / SETAC Europe 22nd Annual Meeting, May 2012, Berlin, Germany. ⟨hal-00704593⟩
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