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Communication Dans Un Congrès Année : 2012

Evaluation of the contamination of aquatic micro-organisms by micro-QuEChERS-nano-LC-MS/MS.

Résumé

It is currently accepted that the main route for pharmaceuticals and Endocrine Disruptors Chemicals (EDCs) to the aquatic environment is via sewage treatment plants receiving wastewater from households, industries and hospitals. In a concern to follow their fates and their impacts in the environment, we developed analytical methods to quantify endogen and synthetic hormones, pharmaceuticals and chemicals in benthic species. We established a molecule list in accordance with prioritisation lists [1] and identification of contamination sources around the wastewater treatment plant (industries, hospital, villages): estrone, 17α-ethinyl-estradiol, lorazepam, oxazepam, acetaminophen, carbamazepine, ibuprofen, 4-methylbenzylidene camphor, fluoroquinolones and sulfonamides for example. We decided to evaluate the contamination of three different species which are well-known bio indicators of water quality. More precisely, invertebrates like gastropods (Potamopyrgus antipodarum), amphipods (Gammarus) and chironomidae larvae (bloodworms) are exposed to effluents from treatment plants.. In the interests of handling a few milligrams of biological matrices and of being accurate, we chose an adequate analytical technique: the innovative nano-LC coupled with tandem mass spectrometer. After cryo-grinding, we extracted samples with a salting-out assisted LLE followed by a purifying dSPE. The extract was evaporated then reconstituted in a proper mobile phase. Only a 1-μl injection is done on the pre-concentration cartridge and the capillary column. Thus, we can reach limits of detection of a few injected femtograms. In conclusion, the comprehensive method was applied to the study of the fate and bio-accumulation of preoccupying micro-pollutants in aquatic micro-organisms.
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Dates et versions

hal-00704524 , version 1 (05-06-2012)

Identifiants

  • HAL Id : hal-00704524 , version 1

Citer

Mikaël Tournier, Julien Camilleri, Robert Baudot, Laure Wiest, Audrey Buleté, et al.. Evaluation of the contamination of aquatic micro-organisms by micro-QuEChERS-nano-LC-MS/MS.. 6th SETAC World Congress / SETAC Europe 22nd Annual Meeting, May 2012, Berlin, Germany. ⟨hal-00704524⟩
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