Synchrotron radiation FTIR detection of a metal-carbonyl tamoxifen analog. Correlation with luminescence microscopy to study its subcellular distribution. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biotechnology Advances Année : 2012

Synchrotron radiation FTIR detection of a metal-carbonyl tamoxifen analog. Correlation with luminescence microscopy to study its subcellular distribution.

Résumé

1,1-Di(4-hydroxyphenyl)-2-cyrhetrenylbut-1-ene 1 is an organometallic conjugate where a [(Cp)Re(CO)(3)] unit is linked to a hydroxytamoxifen-like structure. Its subcellular nuclear distribution was previously observed in a single cell using the near-field technique AFMIR. We show here that synchrotron radiation FTIR spectromicroscopy (SR-FTIR-SM) enabled the mapping of 1 based on its IR-signature (characteristic bands in the 1850-2200cm(-1) range) and pointed out the colocalization of 1 with an area of high amide density. Fluorescence microscopy using DAPI staining performed on the same cells confirmed that this area corresponds to the cell nucleus.

Domaines

Chimie organique

Dates et versions

hal-00680917 , version 1 (20-03-2012)

Identifiants

Citer

Sylvain Clède, François Lambert, Christophe Sandt, Zoher Gueroui, Nicolas Delsuc, et al.. Synchrotron radiation FTIR detection of a metal-carbonyl tamoxifen analog. Correlation with luminescence microscopy to study its subcellular distribution.. Biotechnology Advances, 2012, 51 (3), pp.393-395. ⟨10.1016/j.biotechadv.2012.01.023⟩. ⟨hal-00680917⟩
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