Synchrotron radiation FTIR detection of a metal-carbonyl tamoxifen analog. Correlation with luminescence microscopy to study its subcellular distribution. - Archive ouverte HAL Access content directly
Journal Articles Biotechnology Advances Year : 2012

Synchrotron radiation FTIR detection of a metal-carbonyl tamoxifen analog. Correlation with luminescence microscopy to study its subcellular distribution.

Abstract

1,1-Di(4-hydroxyphenyl)-2-cyrhetrenylbut-1-ene 1 is an organometallic conjugate where a [(Cp)Re(CO)(3)] unit is linked to a hydroxytamoxifen-like structure. Its subcellular nuclear distribution was previously observed in a single cell using the near-field technique AFMIR. We show here that synchrotron radiation FTIR spectromicroscopy (SR-FTIR-SM) enabled the mapping of 1 based on its IR-signature (characteristic bands in the 1850-2200cm(-1) range) and pointed out the colocalization of 1 with an area of high amide density. Fluorescence microscopy using DAPI staining performed on the same cells confirmed that this area corresponds to the cell nucleus.
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Dates and versions

hal-00680917 , version 1 (20-03-2012)

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Sylvain Clède, François Lambert, Christophe Sandt, Zoher Gueroui, Nicolas Delsuc, et al.. Synchrotron radiation FTIR detection of a metal-carbonyl tamoxifen analog. Correlation with luminescence microscopy to study its subcellular distribution.. Biotechnology Advances, 2012, 51 (3), pp.393-395. ⟨10.1016/j.biotechadv.2012.01.023⟩. ⟨hal-00680917⟩
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