Fate mapping analysis of lymphoid cells expressing the NKp46 cell surface receptor. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2011

Fate mapping analysis of lymphoid cells expressing the NKp46 cell surface receptor.

Résumé

NKp46 is a cell surface receptor expressed on natural killer (NK) cells, on a minute subset of T cells, and on a population of innate lymphoid cells that produce IL-22 and express the transcription factor retinoid-related orphan receptor (ROR)-γt, referred to as NK cell receptor (NKR)(+)ROR-γt(+) cells. Here we describe Nkp46(iCre) knock-in mice in which the gene encoding the improved Cre (iCre) recombinase was inserted into the Nkp46 locus. This mouse was used to noninvasively trace cells expressing NKp46 in vivo. Fate mapping experiments demonstrated the stable expression of NKp46 on NK cells and allowed a reappraisal of the sequential steps of NK cell maturation. NKp46 genetic tracing also showed that gut NKR(+)ROR-γt(+) and NK cells represent two distinct lineages. In addition, the genetic heterogeneity of liver NK cells was evidenced. Finally, Nkp46(iCre) mice also represent a unique mouse model of conditional mutagenesis specifically in NKp46(+) cells, paving the way for further developments in the biology of NKp46(+) NK, T, and NKR(+)ROR-γt(+) cells.

Domaines

Immunologie

Dates et versions

hal-00672199 , version 1 (20-02-2012)

Identifiants

Citer

Emilie Narni-Mancinelli, Julie Chaix, Aurore Fenis, Yann M Kerdiles, Nadia Yessaad, et al.. Fate mapping analysis of lymphoid cells expressing the NKp46 cell surface receptor.. Proceedings of the National Academy of Sciences of the United States of America, 2011, 108 (45), pp.18324-9. ⟨10.1073/pnas.1112064108⟩. ⟨hal-00672199⟩
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