1-Pentanol is among the possible alcohols usable as an alternative to conventional gasoline and diesel fuels. In order to better understand the combustion characteristics of 1-pentanol, this study presents new experimental data for 1-pentanol in two complementary experimental configurations. Stable species concentration profiles were measured in a jet-stirred reactor (JSR) at 10 atm over a range of equivalence ratios and temperatures. Laminar flame speeds of 1-pentanol-air premixed laminar flame were measured at 1 atm and 423 K for equivalence ratios of 0.7–1.4. The oxidation of 1-pentanol in these experimental configurations was modeled using an extended detailed chemical kinetic reaction mechanism (2099 reactions involving 261 species) derived from a previously proposed scheme for the oxidation of methanol-, ethanol-, and 1-butanol-based fuels. The proposed mechanism shows good agreement with the present experimental data. Reaction path analyses were conducted to interpret the JSR results.