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              <p>We have developed chemical sensors based on strong modiﬁcations of rubrene nanocrystals ﬂuorescence decays under different chemical surroundings. These nanocrystals were grown in silicate sol-gel thin ﬁlms. To improve the nanosensors selectivity, we used alkoxide mixtures of (TMOS: tetramethoxysilane, MTMOS: methyltrimethoxysilane and TMSE: 1,2-bis(trimethoxysilyl)ethane) to modulate the pore size of the sol-gel ﬁlms. Pore size distributions were determined by ellipsometry coupled to gas adsorption- desorption technique. We showed that matrices prepared with equimolar TMOS/MTMOS mixtures are typically microporous while new matrices obtained from 2TMOS/TMSE mixtures are mesoporous. Time-resolved ﬂuorescence spectroscopy was used to follow the diffusion of typical probe molecules (Methylene Blue and Cibacron Blue) via the porosity of the sol-gel ﬁlms. The ﬂuorescence quenching of nanocrystals is in good agreement with the pore sizes determined by ellipsometric porosimetry. Finally, we have demonstrated the basic working principles of ﬂuorescent organic nanocrystals as chem- ical sensors in aqueous solutions, particularly in the case of H2O2.</p>
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