Clostridium botulinum Group I Strain Genotyping by 15-Locus Multilocus Variable-Number Tandem-Repeat Analysis. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Clinical Microbiology Année : 2011

Clostridium botulinum Group I Strain Genotyping by 15-Locus Multilocus Variable-Number Tandem-Repeat Analysis.

Silvia Fillo
  • Fonction : Auteur
Francesco Giordani
  • Fonction : Auteur
Fabrizio Anniballi
  • Fonction : Auteur
Olivier Gorgé
  • Fonction : Auteur
Vincent Ramisse
  • Fonction : Auteur
Julia M Riehm
  • Fonction : Auteur
Holger C Scholz
  • Fonction : Auteur
Wolf D Splettstoesser
  • Fonction : Auteur
Jasper Kieboom
  • Fonction : Auteur
Jaran-Strand Olsen
  • Fonction : Auteur
Lucia Fenicia
  • Fonction : Auteur
Florigio Lista
  • Fonction : Auteur

Résumé

Clostridium botulinum is a taxonomic designation that encompasses a broad variety of spore-forming, Gram-positive bacteria producing the botulinum neurotoxin (BoNT). C. botulinum is the etiologic agent of botulism, a rare but severe neuroparalytic disease. Fine-resolution genetic characterization of C. botulinum isolates of any BoNT type is relevant for both epidemiological studies and forensic microbiology. A 10-locus multiple-locus variable-number tandem-repeat analysis (MLVA) was previously applied to isolates of C. botulinum type A. The present study includes five additional loci designed to better address proteolytic B and F serotypes. We investigated 79 C. botulinum group I strains isolated from human and food samples in several European countries, including types A (28), B (36), AB (4), and F (11) strains, and 5 nontoxic Clostridium sporogenes. Additional data were deduced from in silico analysis of 10 available fully sequenced genomes. This 15-locus MLVA (MLVA-15) scheme identified 86 distinct genotypes that clustered consistently with the results of amplified fragment length polymorphism (AFLP) and MLVA genotyping in previous reports. An MLVA-7 scheme, a subset of the MLVA-15, performed on a lab-on-a-chip device using a nonfluorescent subset of primers, is also proposed as a first-line assay. The phylogenetic grouping obtained with the MLVA-7 does not differ significantly from that generated by the MLVA-15. To our knowledge, this report is the first to analyze genetic variability among all of the C. botulinum group I serotypes by MLVA. Our data provide new insights into the genetic variability of group I C. botulinum isolates worldwide and demonstrate that this group is genetically highly diverse.

Dates et versions

hal-00656853 , version 1 (05-01-2012)

Identifiants

Citer

Silvia Fillo, Francesco Giordani, Fabrizio Anniballi, Olivier Gorgé, Vincent Ramisse, et al.. Clostridium botulinum Group I Strain Genotyping by 15-Locus Multilocus Variable-Number Tandem-Repeat Analysis.. Journal of Clinical Microbiology, 2011, 49 (12), pp.4252-63. ⟨10.1128/JCM.05396-11⟩. ⟨hal-00656853⟩
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