Impact of rhizosphere factors on cyclic lipopeptide signature from the plant beneficial strain Bacillus amyloliquefaciens S499. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue FEMS Microbiology Ecology Année : 2011

Impact of rhizosphere factors on cyclic lipopeptide signature from the plant beneficial strain Bacillus amyloliquefaciens S499.

Venant Nihorimbere
  • Fonction : Auteur
Hélène Cawoy
  • Fonction : Auteur
Philippe Thonart
  • Fonction : Auteur
Marc Ongena

Résumé

Cyclic lipopeptides of the surfactin, iturin and fengycin families synthesized by plant-associated Bacilli represent an important class of antibiotics since they may be tightly involved in the protective effect of selected strains against phytopathogens. However, their production by Bacillus cells developing on roots under rhizosphere conditions is still poorly understood. In this work, we combined electrospray and imaging mass spectrometry-based approaches to determine the detailed pattern of surfactins, iturins and fengycins produced in planta by B. amyloliquefaciens S499. Very different production rates were observed for the three cLP families. While surfactin accumulated in significant amounts, much lower quantities of iturins and fengycins were detected in the environment of colonized roots in comparison with laboratory medium. In addition, the surfactin pattern produced by strain S499 evolving on roots is enriched in homologues with long fatty acid chains (C15) compared to the one typically secreted under in vitro conditions. Additional experiments revealed that lipopeptide production by root-associated S499 cells is qualitatively and quantitatively dictated by the specific nutritional context of the rhizosphere (exudates enriched in organic acids, oxygen limitation) but also by the formation of biofilm-related structures around root hairs. As surfactins, iturins and fengycins retain specific functions and bioactivities, the biological relevance of their differential production observed in planta is discussed in the context of biocontrol of plant diseases.

Domaines

Chimie organique

Dates et versions

hal-00653939 , version 1 (20-12-2011)

Identifiants

Citer

Venant Nihorimbere, Hélène Cawoy, Alexandre Seyer, Alain Brunelle, Philippe Thonart, et al.. Impact of rhizosphere factors on cyclic lipopeptide signature from the plant beneficial strain Bacillus amyloliquefaciens S499.. FEMS Microbiology Ecology, 2011, 79 (1), pp.176-191. ⟨10.1111/j.1574-6941.2011.01208.x⟩. ⟨hal-00653939⟩
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