Regional differences in porcine adipocytes isolated from skeletal muscle and adipose tissues as identified by a proteomic approach. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Animal Science Année : 2008

Regional differences in porcine adipocytes isolated from skeletal muscle and adipose tissues as identified by a proteomic approach.

Résumé

The content and distribution of body lipids are of special interest for production efficiency and meat quality in the farm animal industry. Triglycerides represent the most variable fraction of tissue lipids, and are mainly stored in adipocytes. Although several studies have reported regional differences in the expression of genes and their products in adipocytes from various species, the characteristics of i.m. adipocytes remain poorly described. To evaluate adipocyte features according to muscle and other fat locations, adipocyte proteins were isolated from trapezius skeletal muscle, and intermuscular, s.c., or perirenal adipose tissues from 6 female pigs (80 d of age). Protein extracts were labeled and analyzed by 2-dimensional, fluorescent, differential gel electrophoresis. The comparisons revealed that 149 spots were always differentially expressed (P < 0.05, ratio exceeding |2|-fold difference) between i.m. adipocytes and the fat cells derived from the 3 other adipose locations. The proteins that were downregulated in i.m. fat cells belonged to various metabolic pathways, such as lipogenesis (cytosolic malate dehydrogenase and isocitrate dehydrogenase, P < 0.01), glycolysis (enolases and aldolase, P

Domaines

Biologie animale
Fichier non déposé

Dates et versions

hal-00653325 , version 1 (19-12-2011)

Identifiants

Citer

Florence Gondret, Nathalie Guitton, C. Guillerm-Regost, Isabelle Louveau. Regional differences in porcine adipocytes isolated from skeletal muscle and adipose tissues as identified by a proteomic approach.. Journal of Animal Science, 2008, 86 (9), pp.2115-2125. ⟨10.2527/jas.2007-0750⟩. ⟨hal-00653325⟩
53 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More