Biochemical characterization of Extracellular Polymeric Substances extracted from an intertidal mudflat using a cation exchange resin. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biochemical Systematics and Ecology Année : 2010

Biochemical characterization of Extracellular Polymeric Substances extracted from an intertidal mudflat using a cation exchange resin.

Résumé

The biochemical characterization of Extracellular Polymeric Substances (EPS) excreted in a European intertidal mudflat (Marennes-Oléron Bay) was performed. Experiments were carried out for the first time in situ, by using an improved extraction recently developed. This innovative procedure, using a cation exchange resin (Dowex), allows separating precisely different fractions of EPS, especially pure bound EPS. Moreover, it avoids the contamination of EPS fractions by residual and intracellular polymers, enabling to properly estimate polymeric contents in each fraction. The results were partly similar to conventional results described in the literature and the amount of colloidal carbohydrates (146µg/g of dry sediment) extracted by the Dowex method fitted well with different EPS estimation in European mudflats. Colloidal carbohydrates were essentially composed of glucose (>50%), a carbon source rapidly consumed by the various communities in the sediment. Pure bound carbohydrates were composed of specific carbohydrates (28% rhamnose, 22% xylose). Residual fractions, considered as containing some refractory bound EPS and mostly other internal polymeric substances, presented a more varied composition rich in carbohydrates: galacturonic acid (20%), mannose (19.5%), glucose (19%), arabinose (15%), xylose (8%), galactose (7%).
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Dates et versions

hal-00652078 , version 1 (15-12-2011)

Identifiants

  • HAL Id : hal-00652078 , version 1

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Guillaume Pierre, Marianne Graber, Francis Orvain, Christine Dupuy, Thierry Maugard. Biochemical characterization of Extracellular Polymeric Substances extracted from an intertidal mudflat using a cation exchange resin.. Biochemical Systematics and Ecology, 2010, 38, pp.917-923. ⟨hal-00652078⟩
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