This paper presents a new experimental approach using PIV technique to measure the local instantaneous laminar burning velocity of a stretched premixed flame. Up to now, from experimental techniques, this physical property was only accessible in average and the instantaneous interactions of flame with flow structures, mixture variations and walls could not be considered. In the present work, the local burning velocity is measured as the difference between the local flame speed and the local fresh gas velocity at the entrance of the flame zone. Two original methods are proposed to deduce these quantities from pair of particle images. The local flame speed is measured from the distance between two successive flame positions. For the flame localization, a new extraction tool combined with a filtering technique is proposed to access to the flame front coordinates with sub-pixel accuracy. The local fresh gas velocity near the flame front is extracted from the maximum of the normal velocity profile, located within 1 mm ahead of the flame front. To achieve the required spatial resolution, a new algorithm based on adaptive interrogation window scheme has been developed by taking into account the flow and flame front topologies. The accuracy and reliability of our developments have been evaluated from two complementary approaches based, respectively, on synthetic images of particle and on the well-established configuration of outwardly propagating spherical flames. In the last part of the paper, an illustration of the potentials of our new approach is shown in the case of a laminar flame propagating through a stratified mixture.