Effects of extracellular products from the pathogenic Vibrio aestuarianus strain 01/32 on lethality and cellular immune responses of the oyster Crassostrea gigas - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Developmental and Comparative Immunology Année : 2006

Effects of extracellular products from the pathogenic Vibrio aestuarianus strain 01/32 on lethality and cellular immune responses of the oyster Crassostrea gigas

Résumé

Vibrio aestuarianus strain 01/32 was previously shown to be pathogenic to Crassostrea gigas juveniles. To investigate virulence mechanisms of this pathogen, we studied the toxicity to oysters of its extracellular products (ECPs). ECPs displayed lethality to animals, with a LD50 value of 3.3 mg/g body weight. To determine the oyster cellular immune responses were induced by these ECPs, we further examined in vitro their effects on C. gigas hemocytes, using flow cytometric-based hemocyte assays. Treatment of hemolymph with ECPs caused a significant inhibition of hemocyte phagocytosis and adhesive capabilities. In contrast, the pathway of reactive oxygen species production was enhanced by higher ECP concentrations. Exposure of hemocytes to live bacteria induced no changes in hemocyte parameters. Together, these results suggest that V. aestuarianus strain 01/32 secretes one or more factors which may play an important role in the pathogenicity of this microorganism, and which display immunosuppressant activities on hemocyte functions.

Dates et versions

hal-00629874 , version 1 (06-10-2011)

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Yannick Labreuche, Philippe Soudant, Madeleine Gonçalves, Christophe Lambert, Jean-Louis Nicolas. Effects of extracellular products from the pathogenic Vibrio aestuarianus strain 01/32 on lethality and cellular immune responses of the oyster Crassostrea gigas. Developmental and Comparative Immunology, 2006, 30 (4), pp.367-379. ⟨10.1016/j.dci.2005.05.003⟩. ⟨hal-00629874⟩
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