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Article Dans Une Revue Biophysical Journal Année : 2011

Linker histones incorporation maintains chromatin fiber plasticity.

Résumé

Genomic DNA in eukaryotic cells is organized in supercoiled chromatin fibers, which undergo dynamic changes during such DNA metabolic processes as transcription or replication. Indeed, DNA-translocating enzymes like polymerases produce physical constraints in vivo. We used single-molecule micromanipulation by magnetic tweezers to study the response of chromatin to mechanical constraints in the same range as those encountered in vivo. We had previously shown that under positive torsional constraints, nucleosomes can undergo a reversible chiral transition toward a state of positive topology. We demonstrate here that chromatin fibers comprising linker histones present a torsional plasticity similar to that of naked nucleosome arrays. Chromatosomes can undergo a reversible chiral transition toward a state of positive torsion (reverse chromatosome) without loss of linker histones.

Domaines

Biophysique

Dates et versions

hal-00625637 , version 1 (22-09-2011)

Identifiants

Citer

Pierre Recouvreux, Christophe Lavelle, Maria Barbi, Natalia Conde E Silva, Eric Le Cam, et al.. Linker histones incorporation maintains chromatin fiber plasticity.. Biophysical Journal, 2011, 100 (11), pp.2726-35. ⟨10.1016/j.bpj.2011.03.064⟩. ⟨hal-00625637⟩
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