<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-00623305</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-22T07:57:18+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Chain-dependent photocytotoxicity of tricationic porphyrin conjugates and related mechanisms of cell death in proliferating human skin keratinocytes</title>
            <author role="aut">
              <persName>
                <forename type="first">João Nuno</forename>
                <surname>Silva</surname>
              </persName>
              <idno type="halauthorid">584143-0</idno>
              <affiliation ref="#struct-142593"/>
              <affiliation ref="#struct-120588"/>
              <affiliation ref="#struct-570078"/>
              <affiliation ref="#struct-300535"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Antoine</forename>
                <surname>Galmiche</surname>
              </persName>
              <email type="md5">0b4ecbb23cf6ffeee34df94c11aba7e6</email>
              <email type="domain">chu-amiens.fr</email>
              <idno type="idhal" notation="numeric">1114820</idno>
              <idno type="halauthorid" notation="string">584103-1114820</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-5836-4646</idno>
              <idno type="IDREF">https://www.idref.fr/059736437</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/FYZ-3341-2022</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/FYZ-3341-2022</idno>
              <affiliation ref="#struct-120588"/>
              <affiliation ref="#struct-570078"/>
              <affiliation ref="#struct-300535"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">João P.C.</forename>
                <surname>Tomé</surname>
              </persName>
              <idno type="halauthorid">584144-0</idno>
              <affiliation ref="#struct-168338"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Agnès</forename>
                <surname>Boullier</surname>
              </persName>
              <email type="md5">2987925fc79442e13f51a54e23a35c3e</email>
              <email type="domain">chu-amiens.fr</email>
              <idno type="idhal" notation="numeric">1112322</idno>
              <idno type="halauthorid" notation="string">584145-1112322</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-3721-3934</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/EOG-7966-2022</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/CHN-1709-2022</idno>
              <idno type="IDREF">https://www.idref.fr/178661783</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/EOG-7966-2022</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/CHN-1709-2022</idno>
              <affiliation ref="#struct-120588"/>
              <affiliation ref="#struct-570078"/>
              <affiliation ref="#struct-300535"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Maria G.P.M.S.</forename>
                <surname>Neves</surname>
              </persName>
              <idno type="halauthorid">584146-0</idno>
              <affiliation ref="#struct-168338"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Eduarda M.P.</forename>
                <surname>Silva</surname>
              </persName>
              <idno type="halauthorid">584147-0</idno>
              <affiliation ref="#struct-168338"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Jean-Claude</forename>
                <surname>Capiod</surname>
              </persName>
              <idno type="idhal" notation="numeric">1431062</idno>
              <idno type="halauthorid" notation="string">584148-1431062</idno>
              <idno type="IDREF">https://www.idref.fr/121592529</idno>
              <affiliation ref="#struct-300535"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">José A.S.</forename>
                <surname>Cavaleiro</surname>
              </persName>
              <idno type="halauthorid">471452-0</idno>
              <affiliation ref="#struct-168338"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">René</forename>
                <surname>Santus</surname>
              </persName>
              <idno type="halauthorid">501584-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Jean-Claude</forename>
                <surname>Mazière</surname>
              </persName>
              <email type="md5">c8a9d543e5ba1f07e3df60ce8e1f9dd8</email>
              <email type="domain">chu-amiens.fr</email>
              <idno type="idhal" notation="numeric">918627</idno>
              <idno type="halauthorid" notation="string">498347-918627</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/FKT-1155-2022</idno>
              <idno type="IDREF">https://www.idref.fr/111284570</idno>
              <affiliation ref="#struct-120588"/>
              <affiliation ref="#struct-570078"/>
              <affiliation ref="#struct-300535"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Paulo</forename>
                <surname>Filipe</surname>
              </persName>
              <idno type="halauthorid">501578-0</idno>
              <affiliation ref="#struct-142593"/>
            </author>
            <author role="crp">
              <persName>
                <forename type="first">Patrice</forename>
                <surname>Morlière</surname>
              </persName>
              <email type="md5">50d2d928fbda206ec4c5d5f94826837b</email>
              <email type="domain">chu-amiens.fr</email>
              <idno type="idhal" notation="numeric">910174</idno>
              <idno type="halauthorid" notation="string">501585-910174</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-5755-9855</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/FNN-4357-2022</idno>
              <idno type="IDREF">https://www.idref.fr/035779772</idno>
              <affiliation ref="#struct-120588"/>
              <affiliation ref="#struct-570078"/>
              <affiliation ref="#struct-300535"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Hal</forename>
                <surname>Peer</surname>
              </persName>
              <email type="md5">8d58fc1b670b8074dd83e4a4c93d5914</email>
              <email type="domain">inria.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2011-09-14 02:56:58</date>
              <date type="whenWritten">2010-06-14</date>
              <date type="whenModified">2026-02-10 03:16:16</date>
              <date type="whenReleased">2011-09-14 02:56:58</date>
              <date type="whenProduced">2010-09-14</date>
              <date type="whenEndEmbargoed">2011-09-14</date>
              <ref type="file" target="https://hal.science/hal-00623305v1/document">
                <date notBefore="2011-09-14"/>
              </ref>
              <ref type="file" subtype="author" n="1" target="https://hal.science/hal-00623305v1/file/PEER_stage2_10.1016%252Fj.bcp.2010.07.033.pdf" id="file-623305-235233">
                <date notBefore="2011-09-14"/>
              </ref>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="148147">
                <persName>
                  <forename>Hal</forename>
                  <surname>Peer</surname>
                </persName>
                <email type="md5">8d58fc1b670b8074dd83e4a4c93d5914</email>
                <email type="domain">inria.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-00623305</idno>
            <idno type="halUri">https://hal.science/hal-00623305</idno>
            <idno type="halBibtex">silva:hal-00623305</idno>
            <idno type="halRefHtml">&lt;i&gt;Biochemical Pharmacology&lt;/i&gt;, 2010, 80 (9), pp.1373. &lt;a target="_blank" href="https://dx.doi.org/10.1016/j.bcp.2010.07.033"&gt;&amp;#x27E8;10.1016/j.bcp.2010.07.033&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Biochemical Pharmacology, 2010, 80 (9), pp.1373. &amp;#x27E8;10.1016/j.bcp.2010.07.033&amp;#x27E9;</idno>
            <availability status="restricted">
              <licence target="https://about.hal.science/hal-authorisation-v1/">HAL Authorization<ref corresp="#file-623305-235233"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="UNIV-PICARDIE">Université de Picardie Jules Verne</idno>
            <idno type="stamp" n="PEER">PEER Publishing and the Ecology of European Research</idno>
            <idno type="stamp" n="U-PICARDIE">Université de Picardie Jules Verne</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Chain-dependent photocytotoxicity of tricationic porphyrin conjugates and related mechanisms of cell death in proliferating human skin keratinocytes</title>
                <author role="aut">
                  <persName>
                    <forename type="first">João Nuno</forename>
                    <surname>Silva</surname>
                  </persName>
                  <idno type="halauthorid">584143-0</idno>
                  <affiliation ref="#struct-142593"/>
                  <affiliation ref="#struct-120588"/>
                  <affiliation ref="#struct-570078"/>
                  <affiliation ref="#struct-300535"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Antoine</forename>
                    <surname>Galmiche</surname>
                  </persName>
                  <email type="md5">0b4ecbb23cf6ffeee34df94c11aba7e6</email>
                  <email type="domain">chu-amiens.fr</email>
                  <idno type="idhal" notation="numeric">1114820</idno>
                  <idno type="halauthorid" notation="string">584103-1114820</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-5836-4646</idno>
                  <idno type="IDREF">https://www.idref.fr/059736437</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/FYZ-3341-2022</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/FYZ-3341-2022</idno>
                  <affiliation ref="#struct-120588"/>
                  <affiliation ref="#struct-570078"/>
                  <affiliation ref="#struct-300535"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">João P.C.</forename>
                    <surname>Tomé</surname>
                  </persName>
                  <idno type="halauthorid">584144-0</idno>
                  <affiliation ref="#struct-168338"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Agnès</forename>
                    <surname>Boullier</surname>
                  </persName>
                  <email type="md5">2987925fc79442e13f51a54e23a35c3e</email>
                  <email type="domain">chu-amiens.fr</email>
                  <idno type="idhal" notation="numeric">1112322</idno>
                  <idno type="halauthorid" notation="string">584145-1112322</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-3721-3934</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/EOG-7966-2022</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/CHN-1709-2022</idno>
                  <idno type="IDREF">https://www.idref.fr/178661783</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/EOG-7966-2022</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/CHN-1709-2022</idno>
                  <affiliation ref="#struct-120588"/>
                  <affiliation ref="#struct-570078"/>
                  <affiliation ref="#struct-300535"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Maria G.P.M.S.</forename>
                    <surname>Neves</surname>
                  </persName>
                  <idno type="halauthorid">584146-0</idno>
                  <affiliation ref="#struct-168338"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Eduarda M.P.</forename>
                    <surname>Silva</surname>
                  </persName>
                  <idno type="halauthorid">584147-0</idno>
                  <affiliation ref="#struct-168338"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Jean-Claude</forename>
                    <surname>Capiod</surname>
                  </persName>
                  <idno type="idhal" notation="numeric">1431062</idno>
                  <idno type="halauthorid" notation="string">584148-1431062</idno>
                  <idno type="IDREF">https://www.idref.fr/121592529</idno>
                  <affiliation ref="#struct-300535"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">José A.S.</forename>
                    <surname>Cavaleiro</surname>
                  </persName>
                  <idno type="halauthorid">471452-0</idno>
                  <affiliation ref="#struct-168338"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">René</forename>
                    <surname>Santus</surname>
                  </persName>
                  <idno type="halauthorid">501584-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Jean-Claude</forename>
                    <surname>Mazière</surname>
                  </persName>
                  <email type="md5">c8a9d543e5ba1f07e3df60ce8e1f9dd8</email>
                  <email type="domain">chu-amiens.fr</email>
                  <idno type="idhal" notation="numeric">918627</idno>
                  <idno type="halauthorid" notation="string">498347-918627</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/FKT-1155-2022</idno>
                  <idno type="IDREF">https://www.idref.fr/111284570</idno>
                  <affiliation ref="#struct-120588"/>
                  <affiliation ref="#struct-570078"/>
                  <affiliation ref="#struct-300535"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Paulo</forename>
                    <surname>Filipe</surname>
                  </persName>
                  <idno type="halauthorid">501578-0</idno>
                  <affiliation ref="#struct-142593"/>
                </author>
                <author role="crp">
                  <persName>
                    <forename type="first">Patrice</forename>
                    <surname>Morlière</surname>
                  </persName>
                  <email type="md5">50d2d928fbda206ec4c5d5f94826837b</email>
                  <email type="domain">chu-amiens.fr</email>
                  <idno type="idhal" notation="numeric">910174</idno>
                  <idno type="halauthorid" notation="string">501585-910174</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-5755-9855</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/FNN-4357-2022</idno>
                  <idno type="IDREF">https://www.idref.fr/035779772</idno>
                  <affiliation ref="#struct-120588"/>
                  <affiliation ref="#struct-570078"/>
                  <affiliation ref="#struct-300535"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">11103</idno>
                <idno type="issn">0006-2952</idno>
                <idno type="eissn">1873-2968</idno>
                <title level="j">Biochemical Pharmacology</title>
                <imprint>
                  <publisher>Elsevier</publisher>
                  <biblScope unit="volume">80</biblScope>
                  <biblScope unit="issue">9</biblScope>
                  <biblScope unit="pp">1373</biblScope>
                  <date type="datePub">2010-09-14</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1016/j.bcp.2010.07.033</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">MAPK pathway</term>
                <term xml:lang="en">PDT</term>
                <term xml:lang="en">photocytotoxicity</term>
                <term xml:lang="en">necrosis</term>
                <term xml:lang="en">autophagy</term>
                <term xml:lang="en">caspases</term>
                <term xml:lang="en">MAPK pathway.</term>
              </keywords>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Photodynamic therapy (PDT) is a poor treatment option for nodular basal cell carcinomas and squamous cell carcinomas. As a result, the search for new photosensitizers with better effectiveness is of current interest. The photocytotoxicity of conjugates (P-R) of a water-soluble tri-cationic porphyrin (P-H) having similar efficiency of production of singlet oxygen, the PDT cytotoxin, has been assessed Links between uptake, intracellular localization, photooxidative stress, photocytotoxicity and ability to induce programmed cell death are established. Conjugates bearing methyl (P-Me), Di-O-isopropylidene-(-D-galactopyranosyl (P-OGal) or -dicyclohexylureidooxycarbonyl (P-DDC) chains are efficiently taken-up by proliferating NCTC 2544 keratinocytes. The relative order of photocytotoxicity is P-OGal &gt;P-DDC=P-Me &gt;&gt; P-H. The photocytotoxic potential of P-Me, P-OGal and P-DDC equals that of endogenous protoporphyrin IX induced by δ-aminolevulinic acid or its esters, the pro-drugs currently employed for PDT of skin lesions. Microfluorometry shows that P-Me, P-OGal, and P-DDC localize in endocytotic or pinocytotic vesicles but not in mitochondria or nucleus. Absence of annexin V binding, caspase activation or chromatin condensation suggests that cell photosensitization by P-R does not induce apoptosis. On the other hand, P-OGal photocytotoxicity correlates with appearance of multiple vesicles that have hallmarks of autophagy compartments, being decorated with the marker LC3 in cells transfected with an expression vector encoding GFP-LC3. p38 and JNK phosphorylation and inhibition of ERK1/2 phosphorylation suggest close relationship between mortality of NCTC 2544 keratinocytes and MAPK pathway impairment. Given their potentially easy formulation, water-soluble P-R are promising powerful photosensitizers for PDT of skin lesions.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-142593" status="VALID">
          <orgName>Faculdade de Medicina [Lisboa]</orgName>
          <desc>
            <address>
              <addrLine>Universidade de Lisboa</addrLine>
              <country key="PT"/>
            </address>
            <ref type="url">https://www.ulisboa.pt/unidade-organica/faculdade-de-medicina</ref>
          </desc>
          <listRelation>
            <relation active="#struct-1114557" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-120588" status="INCOMING">
          <orgName>Inserm</orgName>
          <desc>
            <address>
              <country key="FR"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-303623" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-570078" status="OLD">
          <idno type="IdRef">248687743</idno>
          <idno type="RNSR">201822702C</idno>
          <idno type="ROR">https://ror.org/012h8gy04</idno>
          <orgName>CHirurgie, IMagerie et REgénération tissulaire de l’extrémité céphalique - Caractérisation morphologique et fonctionnelle - UR UPJV 7516</orgName>
          <orgName type="acronym">CHIMERE</orgName>
          <date type="start">2018-01-01</date>
          <date type="end">2025-12-31</date>
          <desc>
            <address>
              <addrLine>Université de Picardie Jules Verne - CURS - CHU Sud avenue Laënnec - 80054 AMIENS CEDEX1</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://chimere.u-picardie.fr</ref>
          </desc>
          <listRelation>
            <relation name="UR7516" active="#struct-300258" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-300535" status="VALID">
          <orgName>CHU Amiens-Picardie</orgName>
          <desc>
            <address>
              <country key="FR"/>
            </address>
          </desc>
        </org>
        <org type="laboratory" xml:id="struct-168338" status="INCOMING">
          <orgName>Department of Chemistry</orgName>
          <desc>
            <address>
              <country key="PT"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-60947" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-1114557" status="VALID">
          <idno type="IdRef">238704718</idno>
          <idno type="ISNI">0000 0001 2181 4263</idno>
          <idno type="ROR">https://ror.org/01c27hj86</idno>
          <idno type="Wikidata">Q1122926</idno>
          <orgName>Universidade de Lisboa = University of Lisbon = Université de Lisbonne [Lisboa]</orgName>
          <orgName type="acronym">ULISBOA</orgName>
          <desc>
            <address>
              <addrLine>Alameda da Universidade 1649-004 Lisboa</addrLine>
              <country key="PT"/>
            </address>
            <ref type="url">https://www.ulisboa.pt/en</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-303623" status="VALID">
          <idno type="IdRef">026388278</idno>
          <idno type="ROR">https://ror.org/02vjkv261</idno>
          <orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
          <orgName type="acronym">INSERM</orgName>
          <desc>
            <address>
              <addrLine>101, rue de Tolbiac, 75013 Paris</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.inserm.fr</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-300258" status="VALID">
          <idno type="IdRef">026403714</idno>
          <idno type="ISNI">0000 0001 0789 1385</idno>
          <idno type="ROR">https://ror.org/01gyxrk03</idno>
          <orgName>Université de Picardie Jules Verne</orgName>
          <orgName type="acronym">UPJV</orgName>
          <desc>
            <address>
              <addrLine>Chemin du Thil - 80000 Amiens</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.u-picardie.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-60947" status="VALID">
          <idno type="ROR">https://ror.org/00nt41z93</idno>
          <orgName>Universidade de Aveiro = University of Aveiro</orgName>
          <desc>
            <address>
              <addrLine>Campus Universitário de Santiago 3810-193 Aveiro</addrLine>
              <country key="PT"/>
            </address>
            <ref type="url">http://www.ua.pt/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>