Study of bioaccumulation and metabolization of fluoxetine in benthic invertebrates by Micro-QuEChERS-NanoLC-Nano-ESI-MS/MS - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2011

Study of bioaccumulation and metabolization of fluoxetine in benthic invertebrates by Micro-QuEChERS-NanoLC-Nano-ESI-MS/MS

Résumé

Fluoxetine is a widely used antidepressant, frequently found in aquatic ecosystems, which presented dissimilar effects on the reproduction of two gastropod species, P. antipodarum and V. piscinalis. Recently it has also been measured in fish downstream of effluent discharges indicating a high bioaccumulation capacity in vertebrate aquatic wildlife. However no data is available on fate and metabolism of pharmaceuticals in invertebrates. To better understand the interspecific sensitivity the bioaccumulation of fluoxetine and its in vivo metabolization into norfluoxetine have to be explored in P. antipodarum and V. piscinalis. This kind of study requires the development of analytical tools for extraction and analysis of traces of fluoxetine and norfluoxetine in biotic environmental matrices like water's benthic invertebrates. These gastropods weigh just a few milligrams. With such a small sample size, extraction step and analysis are more difficult. So, advanced technologies are required to seek drugs traces in complex matrices like gastropods. In this aim, we have established an analytical strategy as which consists in one single extraction for fluoxetine and norfluoxetine based on QuEChERS method, followed by a nano-LC-MSMS analysis. Indeed, nano-chromatography coupled with mass spectrometry (nano-LC-nano-ESI MS/MS) increases sensitivity, reduces the required initial sample amount and is a good tool to answer this ecotoxicological issue. Both gastropods highly bioaccumulated fluoxetine. However, P. antipodarum bioconcentrated more fluoxetine than V. piscinalis, which can only be partly explained by their lipid content. However, N-demethylation into norfluoxetine was similar in both snails, and far less important than in fish. It implied the occurrence of this metabolic pathway in mollusks. The higher sensitivity of P. antipodarum to fluoxetine than V. piscinalis was at least enlightened by its higher bioaccumulation capacity, but not by higher metabolization into an active metabolite.
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Dates et versions

hal-00616357 , version 1 (22-08-2011)

Identifiants

  • HAL Id : hal-00616357 , version 1

Citer

Cécile Cren-Olivé, Audrey Buleté, Robert Baudot, Laure Wiest, Marion Gust, et al.. Study of bioaccumulation and metabolization of fluoxetine in benthic invertebrates by Micro-QuEChERS-NanoLC-Nano-ESI-MS/MS. 21st Annual Meeting of the Society of Environmental Toxicology and Chemistry (SETAC), May 2011, Milan, Italy. ⟨hal-00616357⟩
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