Evidence that ACETATE NON-UTILIZING 1 prevents carbon leakage from peroxisomes during lipid mobilization in Arabidopsis seedlings - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biochemical Journal Année : 2011

Evidence that ACETATE NON-UTILIZING 1 prevents carbon leakage from peroxisomes during lipid mobilization in Arabidopsis seedlings

Elizabeth Allen
  • Fonction : Auteur
Annick Moing
Jonathan Ad Wattis
  • Fonction : Auteur
Tony Larson
  • Fonction : Auteur
Mickael Maucourt
  • Fonction : Auteur
Ian A Graham
  • Fonction : Auteur
Dominique Rolin
  • Fonction : Auteur

Résumé

ACETATE NON-UTILIZING 1 (ACN1) is a short-chain acyl-CoA synthetase, which activates acetate to acetyl-CoA in peroxisomes of Arabidopsis. Pulse-chase [2-13C]acetate feeding of the mutant acn1-2 revealed that acetate accumulation and assimilation were no different to that of wild-type, Col-7. However, the lack of acn1-2 led to a decrease of nearly 50% in 13C-labelling of glutamine, a major carbon sink in seedlings, and large decreases in primary metabolite levels. In contrast, acetyl-CoA levels were higher in acn1-2 compared to Col-7. The disappearance of eicosenoic acid was slightly delayed in acn1-2 indicating only a small effect on the rate of lipid breakdown. A comparison of transcript levels in acn1-2 and col-7 showed that induced genes included a number of metabolic genes and also a large number of signaling-related genes. Genes repressed in the mutant were represented primarily by embryogenesis related genes. Transcript levels of glyoxylate cycle genes also were lower in acn1-2 than in Col-7. We conclude that deficiency in peroxisomal acetate assimilation comprises only a small proportion of total acetate use, but this affects both primary metabolism and gene expression. We discuss the possibility that ACN1 safeguards against the loss of carbon as acetate from peroxisomes during lipid mobilization.

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Dates et versions

hal-00608395 , version 1 (13-07-2011)

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Elizabeth Allen, Annick Moing, Jonathan Ad Wattis, Tony Larson, Mickael Maucourt, et al.. Evidence that ACETATE NON-UTILIZING 1 prevents carbon leakage from peroxisomes during lipid mobilization in Arabidopsis seedlings. Biochemical Journal, 2011, 437 (3), pp.505-513. ⟨10.1042/BJ20101764⟩. ⟨hal-00608395⟩

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