Probing Orientational Order of MHC Class I Protein and Lipids in Cell Membranes by Fluorescence Polarization-Resolved Microscopy Imaging - Archive ouverte HAL Access content directly
Journal Articles Biophysical Journal Year : 2011

Probing Orientational Order of MHC Class I Protein and Lipids in Cell Membranes by Fluorescence Polarization-Resolved Microscopy Imaging

A. Kress
  • Function : Author
Patrick Ferrand
H. Rigneault
S. Brasselet
Connectez-vous pour contacter l'auteur

Abstract

Steady-state polarization-resolved fluorescence imaging is used to analyze the molecular orientational order behavior of rigidly labeled major histocompatibility complex class I (MHC I) proteins and lipid probes in cell membranes of living cells. These fluorescent probes report the orientational properties of proteins and their surrounding lipid environment. We present a statistical study of the molecular orientational order, modeled as the width of the angular distribution of the molecules, for the proteins in the cell endomembrane and plasma membrane, as well as for the lipid probes in the plasma membrane. We apply this methodology on cells after treatments affecting the actin and microtubule networks. We find in particular opposite orientational order changes of proteins and lipid probes in the plasma membrane as a response to the cytoskeleton disruption. This suggests that MHC I orientational order is governed by its interaction with the cytoskeleton, whereas the plasma membrane lipid order is governed by the local cell membrane morphology.
Fichier principal
Vignette du fichier
Kress_BiopphysJ_2011.pdf (6.6 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

hal-00601658 , version 1 (07-10-2014)

Identifiers

Cite

A. Kress, Patrick Ferrand, H. Rigneault, T. Trombik, H.-T. He, et al.. Probing Orientational Order of MHC Class I Protein and Lipids in Cell Membranes by Fluorescence Polarization-Resolved Microscopy Imaging. Biophysical Journal, 2011, 101, pp.468. ⟨10.1016/j.bpj.2011.05.021⟩. ⟨hal-00601658⟩
248 View
129 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More