Intragastric administration of a superoxide dismutase-producing recombinant Lactobacillus casei BL23 strain attenuates DSS colitis in mice. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue International Journal of Food Microbiology Année : 2010

Intragastric administration of a superoxide dismutase-producing recombinant Lactobacillus casei BL23 strain attenuates DSS colitis in mice.

Tatiana Rochat
Claire Cherbuy
Ismael Bouloufa
  • Fonction : Auteur
François Lefèvre
  • Fonction : Auteur
  • PersonId : 1202741
Jean-Jacques Gratadoux
  • Fonction : Auteur
Edith Honvo-Hueto
  • Fonction : Auteur
Sébastien Blugeon
  • Fonction : Auteur
  • PersonId : 1202910
Gérard Corthier
  • Fonction : Auteur
Philippe Langella
  • Fonction : Auteur
Luis G Bermúdez-Humarán
  • Fonction : Auteur

Résumé

Human immune cells release large amounts of reactive oxygen species (ROS) such as superoxide radical and hydrogen peroxide via respiratory burst. In inflammatory bowel diseases, a sustained and abnormal activation of the immune response results in oxidative stress of the digestive tract and in a loss of intestinal homeostasis. We previously reported that heterologous production of the Lactobacillus plantarum manganese catalase (MnKat) enhances the survival of Lb. casei BL23 when exposed to oxidative stress. Anti-inflammatory effects were observed after Lb. casei BL23 oral administrations in moderate murine dextran sodium sulfate (DSS)-induced colitis, without added effects of the MnKat production. Here, we evaluated the protective effects obtained by an improved antioxidative strategy. The Lactococcus lactis sodA gene was expressed in Lb. casei BL23 which acquired an efficient manganese superoxide dismutase (MnSOD) activity. The effects of Lb. casei MnSOD alone or in combination with Lb. casei MnKat were compared first in eukaryotic cell PMA-induced oxidative stress model and then in severe murine DSS-induced colitis. Based on ROS production assays as well as colonic histological scores, a significant reduction of both oxidative stress and inflammation was observed with Lb. casei MnSOD either alone or in combination with Lb. casei MnKat. No added effect of the presence of Lb. casei MnKat was observed. These results suggest that Lb. casei BL23 MnSOD could have anti-inflammatory effects on gut inflammation.

Dates et versions

hal-00600679 , version 1 (15-06-2011)

Identifiants

Citer

Laurie Watterlot, Tatiana Rochat, Harry Sokol, Claire Cherbuy, Ismael Bouloufa, et al.. Intragastric administration of a superoxide dismutase-producing recombinant Lactobacillus casei BL23 strain attenuates DSS colitis in mice.. International Journal of Food Microbiology, 2010, 144 (1), pp.35-41. ⟨10.1016/j.ijfoodmicro.2010.03.037⟩. ⟨hal-00600679⟩
195 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More